ArticleNature biotechnology1998
Engineering passive immunity in transgenic mice secreting virus-neutralizing antibodies in milk.
Article in Nature biotechnology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 85 citations in OpenAlex.
- Heritable immunization of mice against Lyme disease enables ecological disease prevention.Nature communications · 2026Article
- Article
- Anti-SARS-CoV-2-specific antibodies in human breast milk following SARS-CoV-2 infection during pregnancy: a prospective cohort study.International breastfeeding journal · 2024Article
- A Comprehensive Analysis of Maternal and Newborn Disease and Related Control for COVID-19.SN comprehensive clinical medicine · 2021Review
- Applying Immune Instincts and Maternal Intelligence from Comparative Microbiology to COVID-19.SN comprehensive clinical medicine · 2020Review
- Use of transgenic animals in biotechnology: prospects and problems.Acta naturae · 2013Article
- Expression of recombinant antibodies.Frontiers in immunology · 2013Article
- Evaluation of combinatorial cis-regulatory elements for stable gene expression in chicken cells.BMC biotechnology · 2010Article
- Functional recombinant human anti-HAV antibody expressed in milk of transgenic mice.Transgenic research · 2009Article
- Designer milk.Advances in food and nutrition research · 2007Review
- An antibody derivative expressed from viral vectors passively immunizes pigs against transmissible gastroenteritis virus infection when supplied orally in crude plant extracts.Plant biotechnology journal · 2006Article
- Maternally derived recombinant human anti-hantavirus monoclonal antibodies are transferred to mouse offspring during lactation and neutralize virus in vitro.Journal of virology · 2006Article
- Use of transgenic animals to improve human health and animal production.Reproduction in domestic animals = Zuchthygiene · 2005Review
- Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Cloned transgenic farm animals produce a bispecific antibody for T cell-mediated tumor cell killing.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Neutralization of enteric coronaviruses with Escherichia coli cells expressing single-chain Fv-autotransporter fusions.Journal of virology · 2003Article
- 'Molecular farming' of antibodies in plants.Die Naturwissenschaften · 2003Review
- Mammary expression of new genes to combat mastitis.Journal of animal science · 2003Review
- Antibody manufacture in transgenic animals and comparisons with other systems.Current opinion in biotechnology · 2002Review
- The prospects of modifying the antimicrobial properties of milk.Biotechnology advances · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protection against enteric infections can be provided by the oral administration of pathogen-neutralizing antibodies. To provide passive immunity, 18 lines of transgenic mice secreting a recombinant monoclonal antibody (Mab) neutralizing transmissible gastroenteritis coronavirus (TGEV) into the milk were generated. The genes encoding a chimeric Mab with the variable modules of the murine TGEV-specific Mab 6A.C3 and the constant modules of a human IgG, isotype Mab were expressed under the control of regulatory sequences derived from the whey acidic protein, which is an abundant milk protein. The Mab 6A.C3 binds to a highly conserved epitope present in coronaviruses of several species, which does not allow the selection of neutralization escape mutants. Antibody expression titers of 10(6) were obtained in the milk of transgenic mice that reduced TGEV infectivity 10(6)-fold. The antibody was synthesized at high levels throughout lactation. Integration of matrix attachment region sequences with the antibody genes led to a 20- to 10,000-fold increase in the antibody titer in 50% of the transgenic animals. Antibody expression levels were transgene copy number independent and related to the site of integration. The generation of transgenic animals producing virus neutralizing antibodies in milk could provide an approach to protection against neonatal infections of the enteric tract.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.