ArticleThe Plant cell1998
Expression of lauroyl-acyl carrier protein thioesterase in brassica napus seeds induces pathways for both fatty acid oxidation and biosynthesis and implies a set point for triacylglycerol accumulation
Article in The Plant cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 172 citations in OpenAlex.
- Organelle-Targeted Multi-Enzyme Engineering Enables Medium-Chain Fatty Acid Production inJournal of microbiology and biotechnology · 2026Article
- Identification of a promoter region specifically active in the maturing endosperm of Arabidopsis seeds and its use for targeted modification of fatty acid metabolism.The Plant journal : for cell and molecular biology · 2025Article
- How Close Are We to the Production of Milk in Alternative Systems? The Fat Perspective.Foods (Basel, Switzerland) · 2025Review
- Triacylglycerol stability limits futile cycles and inhibition of carbon capture in oil-accumulating leaves.Plant physiology · 2025Article
- Development of vegetative oil sorghum: From lab-to-field.Plant biotechnology journal · 2025Article
- The identification and expression analysis of walnut Acyl-ACP thioesterases.Frontiers in genetics · 2024Article
- Identification and Functional Characterization of Acyl-ACP Thioesterases B (GhFatBs) Responsible for Palmitic Acid Accumulation in Cotton Seeds.International journal of molecular sciences · 2022Article
- Multilevel interactions between native and ectopic isoprenoid pathways affect global metabolism in rice.Transgenic research · 2022Article
- In Silico Analysis of Fatty Acid Desaturases Structures inInternational journal of molecular sciences · 2021Article
- Large fluxes of fatty acids from membranes to triacylglycerol and back during N-deprivation and recovery in Chlamydomonas.Plant physiology · 2021Article
- Overexpression of Acyl-ACP Thioesterases,International journal of molecular sciences · 2019Article
- Cellular and Molecular Responses ofFrontiers in microbiology · 2018Article
- Article
- A reconfigured Kennedy pathway which promotes efficient accumulation of medium-chain fatty acids in leaf oils.Plant biotechnology journal · 2017Article
- Engineering the production of conjugated fatty acids in Arabidopsis thaliana leaves.Plant biotechnology journal · 2017Article
- Vegetable Oil: Nutritional and Industrial Perspective.Current genomics · 2016Article
- Genome-Wide Association Study of Arabidopsis thaliana Identifies Determinants of Natural Variation in Seed Oil Composition.The Journal of heredity · 2016Article
- Article
- Current progress towards the metabolic engineering of plant seed oil for hydroxy fatty acids production.Plant cell reports · 2015Review
- Fatty acid synthesis is inhibited by inefficient utilization of unusual fatty acids for glycerolipid assembly.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of a California bay lauroyl-acyl carrier protein thioesterase (MCTE) in developing seeds of transgenic oilseed rape alters the fatty acid composition of the mature seed, resulting in up to 60 mol% of laurate in triacylglycerols. In this study, we examined the metabolism of lauric acid and 14C-acetate in developing seeds of oilseed rape that express high levels of MCTE. Lauroyl-CoA oxidase activity but not palmitoyl-CoA oxidase activity was increased several-fold in developing seeds expressing MCTE. In addition, isocitrate lyase and malate synthase activities were six- and 30-fold higher, respectively, in high-laurate developing seeds. Control seeds incorporated 14C-acetate almost entirely into fatty acids, whereas in seeds expressing MCTE, only 50% of the label was recovered in lipids and the remainder was in a range of water-soluble components, including sucrose and malate. Together, these results indicate that the pathways for beta-oxidation and the glyoxylate cycle have been induced in seeds expressing high levels of MCTE. Although a substantial portion of the fatty acid produced in these seeds is recycled to acetyl-CoA and sucrose through the beta-oxidation and glyoxylate cycle pathways, total seed oil is not reduced. How is oil content maintained if lauric acid is inefficiently converted to triacylglycerol? The levels of acyl carrier protein and several enzymes of fatty acid synthesis were increased two- to threefold at midstage development in high-laurate seeds. These results indicate that a coordinate induction of the fatty acid synthesis pathway occurs, presumably to compensate for the lauric acid lost through beta-oxidation or for a shortage of long-chain fatty acids.
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Registered trials
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