ArticleThe Journal of physiology1998
Ionotropic and metabotropic activation of a neuronal chloride channel by serotonin and dopamine in the leech Hirudo medicinalis.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 27 citations in OpenAlex.
- Circuit-constrained molecular neuroethology in the medicinal leech Hirudo verbana.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Review
- The dynamics of group formation among leeches.Frontiers in physiology · 2012Article
- Behavioral choice by presynaptic inhibition of tactile sensory terminals.Nature neuroscience · 2009Article
- Effects of calcium channel blockers on antidepressant action of Alprazolam and Imipramine.The Libyan journal of medicine · 2007Article
- Non-associative learning and serotonin induce similar bi-directional changes in excitability of a neuron critical for learning in the medicinal leech.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Direct and indirect regulation of a single ion channel.The Journal of physiology · 1998Article
Corrections and comments
- Commented on by
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Cl- channels on the pressure-sensitive (P) neuron in the leech are directly activated by synaptic release of serotonin (5-HT) and are indirectly stimulated by the cAMP second messenger pathway, suggesting an unusual dual regulation of the channels. We have investigated the mode of action of 5-HT and dopamine (DA) on a Cl- channel in adult P cells in culture by recording from cell-attached patches. 2. 5-HT increased Cl- channel activity only when included in the recording pipette and not when applied in the bath. 3. Pipette or, more effectively, bath application of DA led to an increase in Cl- channel activity. This effect was blocked by the potent and specific dopaminergic (DA1) receptor blocker, SCH-23390. 4. The stimulation by DA, but not by 5-HT, was also blocked by the cAMP-dependent protein kinase A (PKA) inhibitor Rp-cAMP and was mimicked by the membrane-permeant cAMP analogue dibutyryl cAMP (db-cAMP). 5. Our results show that 5-HT directly gates a Cl- channel that is also activated by DA via the cAMP pathway. This study demonstrates that a ligand-gated channel can be independently operated by another transmitter acting via a second messenger pathway.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.