ArticleNucleic acids research1998
Short unligated sticky ends enable the observation of circularised DNA by atomic force and electron microscopies.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Single molecule detection of PARP1 and PARP2 interaction with DNA strand breaks and their poly(ADP-ribosyl)ation using high-resolution AFM imaging.Nucleic acids research · 2016Article
- High-resolution AFM imaging of single-stranded DNA-binding (SSB) protein--DNA complexes.Nucleic acids research · 2007Article
- Studying the effect of a charged surface on the interaction of bleomycin with DNA using an atomic force microscope.European biophysics journal : EBJ · 2005Article
- Adsorption of DNA to mica mediated by divalent counterions: a theoretical and experimental study.Biophysical journal · 2003Article
- Recognition of the DNA sequence by an inorganic crystal surface.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Shear-induced assembly of lambda-phage DNA.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A comparative study of the stabilisation of DNA sticky ends by divalent cations was carried out by atomic force microscopy (AFM), electron microscopy and agarose gel electrophoresis. At room temperature, molecules bearing such extremities are immediately oligomerised or circularised by addition of Mg2+or Ca2+. This phenomenon, more clearly detected by AFM, requires the presence of uranyl salt, which stabilises the structures induced by Mg2+or Ca2+. DNA fragments were obtained by restriction enzymes producing sticky ends of 2 or 4 nucleotides (nt) in length with different guanine plus cytosine (GC) contents. The stability of the pairing is high when ends of 4 nt display a 100% GC-content. In that case, 95% of DNA fragments are maintained circular by the divalent cations, although 2 nt GC-sticky ends are sufficient for a stable pairing. DNA fragments with one blunt end and the other sticky appear as dimers in the presence of Mg2+. Dimerisation was analysed by varying the lengths and concentrations of DNA fragments, the base composition of the sticky ends, and also the temperature. Our observation provides a new powerful tool for construction of inverted dimers, and circularisation, ligation analysis or short bases sequence interaction studies.
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