ArticleApplied and environmental microbiology1998
Development of a direct in situ PCR method for detection of specific bacteria in natural environments.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 83 citations in OpenAlex.
- Single-Cell RNA Sequencing of Plant-Associated Bacterial Communities.Frontiers in microbiology · 2019Review
- High-frequency phage-mediated gene transfer among Escherichia coli cells, determined at the single-cell level.Applied and environmental microbiology · 2007Article
- Proposed model: mechanisms of immunomodulation induced by probiotic bacteria.Clinical and vaccine immunology : CVI · 2007Review
- Quantitative determination of free-DNA uptake in river bacteria at the single-cell level by in situ rolling-circle amplification.Applied and environmental microbiology · 2006Article
- Visualization and enumeration of bacteria carrying a specific gene sequence by in situ rolling circle amplification.Applied and environmental microbiology · 2005Article
- Recognition of individual genes in diverse microorganisms by cycling primed in situ amplification.Applied and environmental microbiology · 2005Article
- rRNA sequence-based scanning electron microscopic detection of bacteria.Applied and environmental microbiology · 2005Article
- Single-cell microbiology: tools, technologies, and applications.Microbiology and molecular biology reviews : MMBR · 2004Review
- Two bacteria phylotypes are predominant in the Suiyo seamount hydrothermal plume.Applied and environmental microbiology · 2004Article
- Detection of bacteria carrying the stx2 gene by in situ loop-mediated isothermal amplification.Applied and environmental microbiology · 2003Article
- Monitoring of Ralstonia eutropha KT1 in groundwater in an experimental bioaugmentation field by in situ PCR.Applied and environmental microbiology · 2002Article
- Direct detection by in situ PCR of the amoA gene in biofilm resulting from a nitrogen removal process.Applied and environmental microbiology · 2001Article
- Application of sequence-specific labeled 16S rRNA gene oligonucleotide probes for genetic profiling of cyanobacterial abundance and diversity by array hybridization.Applied and environmental microbiology · 2000Article
- Simultaneous direct counting of total and specific microbial cells in seawater, using a deep-sea microbe as target.Applied and environmental microbiology · 2000Article
- Diversity of dissimilatory bisulfite reductase genes of bacteria associated with the deep-sea hydrothermal vent polychaete annelid Alvinella pompejana.Applied and environmental microbiology · 1999Article
- Microbiological safety of drinking water: United States and global perspectives.Environmental health perspectives · 1999Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We applied HNPP (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) to direct in situ PCR for the routine detection of specific bacterial cells at the single-cell level. PCR was performed on glass slides with digoxigenin-labeled dUTP. The digoxigenin-labeled PCR products were detected with alkaline phosphatase-labeled antidigoxigenin antibody and HNPP which was combined with Fast Red TR. A bright red fluorescent signal was produced from conversion to HNP (dephosphorylated form) by alkaline phosphatase. We used the ECOL DNA primer set for amplification of ribosomal DNA of Escherichia coli to identify cells specifically at the single-cell level in a bacterial mixture. High-contrast images were obtained under an epifluorescence microscope with in situ PCR. By image analysis, E. coli cells in polluted river water also were detected.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.