ArticleApplied and environmental microbiology1998
Pichia stipitis genes for alcohol dehydrogenase with fermentative and respiratory functions.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 76 citations in OpenAlex.
- Oxygenation influences xylose fermentation and gene expression in the yeast genera Spathaspora and Scheffersomyces.Biotechnology for biofuels and bioproducts · 2024Article
- Review
- Smokeless tobacco consumption induces dysbiosis of oral mycobiome: a pilot study.Applied microbiology and biotechnology · 2022Article
- Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).Journal of industrial microbiology & biotechnology · 2020Review
- Evidence for loss and reacquisition of alcoholic fermentation in a fructophilic yeast lineage.eLife · 2018Article
- Aadh2p: an Arxula adeninivorans alcohol dehydrogenase involved in the first step of the 1-butanol degradation pathway.Microbial cell factories · 2016Article
- Scheffersomyces stipitis: a comparative systems biology study with the Crabtree positive yeast Saccharomyces cerevisiae.Microbial cell factories · 2012Article
- A constraint-based model of Scheffersomyces stipitis for improved ethanol production.Biotechnology for biofuels · 2012Article
- Xylitol production from DEO hydrolysate of corn stover by Pichia stipitis YS-30.Journal of industrial microbiology & biotechnology · 2011Article
- Article
- Pichia stipitis genomics, transcriptomics, and gene clusters.FEMS yeast research · 2009Review
- Efficient production of L-lactic acid from xylose by Pichia stipitis.Applied and environmental microbiology · 2007Article
- Molecular cloning and characterization of the alcohol dehydrogenase ADH1 gene of Candida utilis ATCC 9950.Journal of industrial microbiology & biotechnology · 2006Article
- Molecular cloning of XYL3 (D-xylulokinase) from Pichia stipitis and characterization of its physiological function.Applied and environmental microbiology · 2002Article
- Transcriptional control of ADH genes in the xylose-fermenting yeast Pichia stipitis.Applied and environmental microbiology · 1999Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two genes coding for isozymes of alcohol dehydrogenase (ADH); designated PsADH1 and PsADH2, have been identified and isolated from Pichia stipitis CBS 6054 genomic DNA by Southern hybridization to Saccharomyces cerevisiae ADH genes, and their physiological roles have been characterized through disruption. The amino acid sequences of the PsADH1 and PsADH2 isozymes are 80.5% identical to one another and are 71.9 and 74.7% identical to the S. cerevisiae ADH1 protein. They also show a high level identity with the group I ADH proteins from Kluyveromyces lactis. The PsADH isozymes are presumably localized in the cytoplasm, as they do not possess the amino-terminal extension of mitochondrion-targeted ADHs. Gene disruption studies suggest that PsADH1 plays a major role in xylose fermentation because PsADH1 disruption results in a lower growth rate and profoundly greater accumulation of xylitol. Disruption of PsADH2 does not significantly affect ethanol production or aerobic growth on ethanol as long as PsADH1 is present. The PsADH1 and PsADH2 isozymes appear to be equivalent in the ability to convert ethanol to acetaldehyde, and either is sufficient to allow cell growth on ethanol. However, disruption of both genes blocks growth on ethanol. P. stipitis strains disrupted in either PsADH1 or PsADH2 still accumulate ethanol, although in different amounts, when grown on xylose under oxygen-limited conditions. The PsADH double disruptant, which is unable to grow on ethanol, still produces ethanol from xylose at about 13% of the rate seen in the parental strain. Thus, deletion of both PsADH1 and PsADH2 blocks ethanol respiration but not production, implying a separate path for fermentation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.