ArticleThe EMBO journal1998
Cell wall integrity modulates RHO1 activity via the exchange factor ROM2.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed, 194 citations in OpenAlex.
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- UPR deficiency in budding yeast reveals a trade-off between ER folding capacity and maintenance of euploidy.bioRxiv : the preprint server for biology · 2024Article
- The interplay between cell wall integrity and cell cycle progression in plants.Plant molecular biology · 2023Review
- Bioconversion of rice straw by Lentinula edodes under different spawn formulations.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Yeast Deletomics to Uncover Gadolinium Toxicity Targets and Resistance Mechanisms.Microorganisms · 2023Article
- A comprehensive review and comparison of L-tryptophan biosynthesis inFrontiers in bioengineering and biotechnology · 2023Review
- Silencing ofJournal of fungi (Basel, Switzerland) · 2022Article
- Small GTPase FoSec4-Mediated Protein Secretion Is Important for Polarized Growth, Reproduction and Pathogenicity in the Banana Fusarium Wilt FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Magnaporthe oryzae Chloroplast Targeting Endo-β-1,4-Xylanase I MoXYL1A Regulates Conidiation, Appressorium Maturation and Virulence of the Rice Blast Fungus.Rice (New York, N.Y.) · 2022Article
- Systematic Identification of Essential Genes Required for Yeast Cell Wall Integrity: Involvement of the RSC Remodelling Complex.Journal of fungi (Basel, Switzerland) · 2022Article
- The citron homology domain as a scaffold for Rho1 signaling.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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- Rezafungin-Mechanisms of Action, Susceptibility and Resistance: Similarities and Differences with the Other Echinocandins.Journal of fungi (Basel, Switzerland) · 2020Review
- The Bax inhibitor UvBI-1, a negative regulator of mycelial growth and conidiation, mediates stress response and is critical for pathogenicity of the rice false smut fungus Ustilaginoidea virens.Current genetics · 2019Article
- Integrins in disguise - mechanosensors inMicrobial cell (Graz, Austria) · 2019Review
- Tryptophan confers resistance to SDS-associated cell membrane stress in Saccharomyces cerevisiae.PloS one · 2019Article
- A new cell cycle checkpoint that senses plasma membrane/cell wall damage in budding yeast.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
29 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The essential phosphatidylinositol kinase homologue TOR2 of Saccharomyces cerevisiae controls the actin cytoskeleton by activating a GTPase switch consisting of RHO1 (GTPase), ROM2 (GEF) and SAC7 (GAP). We have identified two mutations, rot1-1 and rot2-1, that suppress the loss of TOR2 and are synthetic-lethal. The wild-type ROT1 and ROT2 genes and a multicopy suppressor, BIG1, were isolated by their ability to rescue the rot1-1 rot2-1 double mutant. ROT2 encodes glucosidase II, and ROT1 and BIG1 encode novel proteins. We present evidence that cell wall defects activate RHO1. First, rot1, rot2, big1, cwh41, gas1 and fks1 mutations all confer cell wall defects and suppress tor2(ts). Second, destabilizing the cell wall by supplementing the growth medium with 0.005% SDS also suppresses a tor2(ts) mutation. Third, disturbing the cell wall with SDS or a rot1, rot2, big1, cwh41, gas1 or fks1 mutation increases GDP/GTP exchange activity toward RHO1. These results suggest that cell wall defects suppress a tor2 mutation by activating RHO1 independently of TOR2, thereby inducing TOR2-independent polarization of the actin cytoskeleton and cell wall synthesis. Activation of RHO1, a subunit of the cell wall synthesis enzyme glucan synthase, by a cell wall alteration would ensure that cell wall synthesis occurs only when and where needed. The mechanism of RHO1 activation by a cell wall alteration is via the exchange factor ROM2 and could be analogous to signalling by integrin receptors in mammalian cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.