ArticleBiophysical journal1998
Depolarization increases the single-channel conductance and the open probability of crayfish glutamate channels.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Sensing membrane voltage by reorientation of dipolar transmembrane peptides.Biophysical journal · 2024Article
- The spatial pattern of light determines the kinetics and modulates backpropagation of optogenetic action potentials.Journal of computational neuroscience · 2013Article
- Effect of membrane tension on the electric field and dipole potential of lipid bilayer membrane.Biochimica et biophysica acta · 2011Article
- Meet me on the other side: trans-bilayer modulation of a model voltage-gated ion channel activity by membrane electrostatics asymmetry.PloS one · 2011Article
- Gating modes in AMPA receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Voltage-dependent gating of NR1/2B NMDA receptors.The Journal of physiology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the voltage sensitivity of glutamate receptors in outside-out patches taken from crayfish muscles. We found that single-channel conductance, measured directly at the single-channel level, increases as depolarization rises. At holding potentials from -90 mV to approximately 20 mV, the conductance is 109 pS. At holding potentials positive to 20 mV, the conductance is 213 pS. This increase in single-channel conductance was also observed in cell-attached patches. In addition, desensitization, rise time, and the dose-response curve were all affected by depolarization. To further clarify these multifaceted effects, we evaluated the kinetic properties of single-channel activity recorded from cell-attached patches in hyperpolarization (membrane potential around -75 mV) and depolarization (membrane potential approximately 105 mV). We found that the glutamate dissociation rate constant (k_) was affected most significantly by membrane potential; it declined 6.5-fold under depolarization. The rate constant of channel closing (k(c)) was also significantly affected; it declined 1.8-fold. The rate constant of channel opening (k(o)) declined only 1.2-fold. The possible physiological significance of the depolarization-mediated changes in the above rate constants is discussed.
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