ArticleJournal of clinical microbiology1998
Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed.
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- Investigation of Various Toxigenic Genes and Antibiotic and Disinfectant Resistance Profiles ofFoods (Basel, Switzerland) · 2024Article
- Characterization of Staphylococcus aureus isolated from milk samples for their virulence, biofilm, and antimicrobial resistance.Scientific reports · 2024Article
- SaLTy: a novelMicrobial genomics · 2024Article
- Characterization of chicken eggs associatedAnimal biotechnology · 2023Article
- Molecular and Phenotypic Characterization of Ocular Methicillin-Resistant Staphylococcus epidermidis Isolates in Taiwan.Investigative ophthalmology & visual science · 2023Article
- Genomic Characterization ofAnimals : an open access journal from MDPI · 2023Article
- In Vivo Pathogenicity of Methicillin-SusceptibleLife (Basel, Switzerland) · 2022Article
- Prevalence, Infectious Characteristics and Genetic Diversity ofPathogens (Basel, Switzerland) · 2022Article
- Evaluation of aminoglycoside modifying enzymes, SCCmec,Heliyon · 2022Article
- Clonal Diversity and Epidemiological Characteristics of ST239-MRSA Strains.Frontiers in cellular and infection microbiology · 2022Article
- Occurrence, distribution and pattern analysis of methicillin resistant (MRSA) and methicillin sensitive (MSSA) Staphylococcus aureus on fomites in public facilities.Pathogens and global health · 2021Article
- Genotypic Characterization of Clinical Isolates ofPathogens (Basel, Switzerland) · 2021Article
- Virulence Factors inAntibiotics (Basel, Switzerland) · 2021Article
- High prevalence nasal carriage of methicillin-resistantInfection prevention in practice · 2021Article
- Article
- Bovine subclinical mastitis-associated methicillin-resistantIranian journal of microbiology · 2021Article
- Characterization of methicillin-resistant3 Biotech · 2020Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A typing procedure for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the product. All coagulase-positive staphylococci produced a single PCR amplification product of either 875, 660, 603, or 547 bp. Those strains of epidemic methicillin-resistant S. aureus 16 (EMRSA-16) studied all gave a product of 547 bp. PCR products were digested with AluI and CfoI, and the fragments were separated by gel electrophoresis. Ten distinct RFLP patterns were found among 85 isolates of methicillin-resistant S. aureus (MRSA) and 10 propagating strains (PS) of methicillin-sensitive S. aureus (MSSA) examined. RFLP patterns 1, 2, and 3 were specific to strains of EMRSA-3, -15, and -16, respectively. By contrast, RFLP patterns 4 and 5 were seen with a heterogeneous collection of strains, together with drug-resistant forms of S. aureus isolated in Europe and four propagating strains used for the international phage set. RFLP pattern 6 was given by the Airedale isolate and PS 95. RFLP pattern 7 encompassed EMRSA-2 (isolate 331), PS 94, and PS 96. An isolate from Germany gave RFLP pattern 8. Eight strains of MSSA gave patterns similar to those of methicillin-resistant strains (RFLP patterns 3, 4, 5, 6, and 7), but two, PS 42E and PS 71, gave unique RFLP patterns 9 and 10, respectively. The coagulase gene PCR products for 24 isolates of MRSA and two isolates of MSSA were sequenced for both strands. The sequences were aligned, and evolutionary lineages were inferred based on pairwise distances between isolates.
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