ArticleJournal of clinical microbiology1998
Identification of a new DNA region specific for members of Mycobacterium tuberculosis complex.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 68 citations in OpenAlex.
- Post-Vaccination Disseminated Bacillus Calmette Guerin Infection Among Children in Southern Iran.Jundishapur journal of microbiology · 2015Article
- Genetic markers, genotyping methods & next generation sequencing in Mycobacterium tuberculosis.The Indian journal of medical research · 2015Article
- senX3-independent contribution of regX3 to Mycobacterium tuberculosis virulence.BMC microbiology · 2014Article
- Differential regulation of the two-component regulatory system senX3-regX3 in Mycobacterium tuberculosis.Microbiology (Reading, England) · 2014Article
- A comparison between the efficiency of the Xpert MTB/RIF assay and nested PCR in identifying Mycobacterium tuberculosis during routine clinical practice.Journal of thoracic disease · 2014Article
- Association of gyrA/B mutations and resistance levels to fluoroquinolones in clinical isolates of Mycobacterium tuberculosis.Emerging microbes & infections · 2014Article
- First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains.Journal of clinical microbiology · 2012Article
- Adaptation to environmental stimuli within the host: two-component signal transduction systems of Mycobacterium tuberculosis.Microbiology and molecular biology reviews : MMBR · 2011Review
- Characterization of the major formamidopyrimidine-DNA glycosylase homolog in Mycobacterium tuberculosis and its linkage to variable tandem repeats.FEMS immunology and medical microbiology · 2009Article
- A single-step sequencing method for the identification of Mycobacterium tuberculosis complex species.PLoS neglected tropical diseases · 2008Article
- Use of PCR-based Mycobacterium tuberculosis genotyping to prioritize tuberculosis outbreak control activities.Journal of clinical microbiology · 2008Article
- Tuberculosis (Mycobacterium microti) in wild field vole populations.Parasitology · 2008Article
- Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis.Journal of clinical microbiology · 2006Article
- Molecular analysis of clinical isolates of Mycobacterium bovis recovered from humans in Italy.Journal of clinical microbiology · 2006Article
- Molecular epidemiology of tuberculosis: current insights.Clinical microbiology reviews · 2006Review
- Discriminatory power and reproducibility of novel DNA typing methods for Mycobacterium tuberculosis complex strains.Journal of clinical microbiology · 2005Article
- Genotyping Mycobacterium ulcerans and Mycobacterium marinum by using mycobacterial interspersed repetitive units.Journal of bacteriology · 2005Article
- Molecular characteristics of strains of the cameroon family, the major group of Mycobacterium tuberculosis in a country with a high prevalence of tuberculosis.Journal of clinical microbiology · 2004Article
- Use of the hupB gene encoding a histone-like protein of Mycobacterium tuberculosis as a target for detection and differentiation of M. tuberculosis and M. bovis.Journal of clinical microbiology · 2004Article
- Genotypic analysis of Mycobacterium tuberculosis in Bangladesh and prevalence of the Beijing strain.Journal of clinical microbiology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The successful use of DNA amplification for the detection of tuberculous mycobacteria crucially depends on the choice of the target sequence, which ideally should be present in all tuberculous mycobacteria and absent from all other bacteria. In the present study we developed a PCR procedure based on the intergenic region (IR) separating two genes encoding a recently identified mycobacterial two-component system named SenX3-RegX3. The senX3-regX3 IR is composed of a novel type of repetitive sequence, called mycobacterial interspersed repetitive units (MIRUs). In a survey of 116 Mycobacterium tuberculosis strains characterized by different IS6110 restriction fragment length polymorphisms, 2 Mycobacterium africanum strains, 3 Mycobacterium bovis strains (including 2 BCG strains), and 1 Mycobacterium microti strain, a specific PCR fragment was amplified in all cases. This collection included M. tuberculosis strains that lack IS6110 or mtp40, two target sequences that have previously been used for the detection of M. tuberculosis. No PCR fragment was amplified when DNA from other organisms was used, giving a sensitivity of 100% and a specificity of 100% in the confidence limit of this study. The numbers of MIRUs were found to vary among strains, resulting in six different groups of strains on the basis of the size of the amplified PCR fragment. However, the vast majority of the strains (approximately 90%) fell within the same group, containing two 77-bp MIRUs followed by one 53-bp MIRU.
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