ArticleProceedings of the National Academy of Sciences of the United States of America1998
Membrane voltage initiates Ca2+ waves and potentiates Ca2+ increases with abscisic acid in stomatal guard cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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Who cites it
91 citing papers in PubMed, 245 citations in OpenAlex.
- Differential regulation of calcium-activated plant kinases in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2025Article
- Potassium homeostasis and signalling: from the whole plant to the subcellular level.Quantitative plant biology · 2025Review
- A charged existence: A century of transmembrane ion transport in plants.Plant physiology · 2024Review
- OnGuard3e: A predictive, ecophysiology-ready tool for gas exchange and photosynthesis research.Plant, cell & environment · 2023Article
- Stomatal Responses of Two Drought-Tolerant Barley Varieties with Different ROS Regulation Strategies under Drought Conditions.Antioxidants (Basel, Switzerland) · 2023Article
- Phosphorylation of the plasma membrane H+-ATPase AHA2 by BAK1 is required for ABA-induced stomatal closure in Arabidopsis.The Plant cell · 2022Article
- Green Tea Catechins, (-)-Catechin Gallate, and (-)-Gallocatechin Gallate are Potent Inhibitors of ABA-Induced Stomatal Closure.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Knockdown ofFrontiers in plant science · 2022Article
- Structural and Functional Insights into the Role of Guard Cell Ion Channels in Abiotic Stress-Induced Stomatal Closure.Plants (Basel, Switzerland) · 2021Review
- The signatures of organellar calcium.Plant physiology · 2021Review
- Guard cell endomembrane CaNature plants · 2021Article
- Membrane voltage as a dynamic platform for spatiotemporal signaling, physiological, and developmental regulation.Plant physiology · 2021Article
- A role for calcium-dependent protein kinases in differential COThe New phytologist · 2021Article
- SAUR proteins and PP2C.D phosphatases regulate H+-ATPases and K+ channels to control stomatal movements.Plant physiology · 2021Article
- Article
- CIPK23 regulates blue light-dependent stomatal opening in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2020Article
- Stomatal immunity against fungal invasion comprises not only chitin-induced stomatal closure but also chitosan-induced guard cell death.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Calcium signals in guard cells enhance the efficiency by which abscisic acid triggers stomatal closure.The New phytologist · 2019Article
- Calcium signals are necessary to establish auxin transporter polarity in a plant stem cell niche.Nature communications · 2019Article
- Guard Cell Membrane Anion Transport Systems and Their Regulatory Components: An Elaborate Mechanism Controlling Stress-Induced Stomatal Closure.Plants (Basel, Switzerland) · 2019Review
31 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In higher plants changes and oscillations in cytosolic free Ca2+ concentration ([Ca2+]i) are central to hormonal physiology, including that of abscisic acid (ABA), which signals conditions of water stress and alters ion channel activities in guard cells of higher-plant leaves. Such changes in [Ca2+]i are thought to encode for cellular responses to different stimuli, but their origins and functions are poorly understood. Because transients and oscillations in membrane voltage also occur in guard cells and are elicited by hormones, including ABA, we suspected a coupling of [Ca2+]i to voltage and its interaction with ABA. We recorded [Ca2+]i by Fura2 fluorescence ratio imaging and photometry while bringing membrane voltage under experimental control with a two-electrode voltage clamp in intact Vicia guard cells. Free-running oscillations between voltages near -50 mV and -200 mV were associated with oscillations in [Ca2+]i, and, under voltage clamp, equivalent membrane hyperpolarizations caused [Ca2+]i to increase, often in excess of 1 microM, from resting values near 100 nM. Image analysis showed that the voltage stimulus evoked a wave of high [Ca2+]i that spread centripetally from the peripheral cytoplasm within 5-10 s and relaxed over 40-60 s thereafter. The [Ca2+]i increases showed a voltage threshold near -120 mV and were sensitive to external Ca2+ concentration. Substituting Mn2+ for Ca2+ to quench Fura2 fluorescence showed that membrane hyperpolarization triggered a divalent influx. ABA affected the voltage threshold for the [Ca2+]i rise, its amplitude, and its duration. In turn, membrane voltage determined the ability of ABA to raise [Ca2+]i. These results demonstrate a capacity for voltage to evoke [Ca2+]i increases, they point to a dual interaction with ABA in triggering and propagating [Ca2+]i increases, and they implicate a role for voltage in "conditioning" [Ca2+]i signals that regulate ion channels for stomatal function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.