ArticleProceedings of the National Academy of Sciences of the United States of America1998
A novel glutamine-RNA interaction identified by screening libraries in mammalian cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
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- Molecular and genetic characterization of natural HIV-1 Tat Exon-1 variants from North India and their functional implications.PloS one · 2014Article
- The arginine-rich RNA-binding motif of HIV-1 Rev is intrinsically disordered and folds upon RRE binding.Biophysical journal · 2013Article
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- A cell-based method for screening RNA-protein interactions: identification of constitutive transport element-interacting proteins.PloS one · 2012Article
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- Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses.The Journal of general virology · 2011Article
- One ancestor for two codes viewed from the perspective of two complementary modes of tRNA aminoacylation.Biology direct · 2009Article
- Targeting tat inhibitors in the assembly of human immunodeficiency virus type 1 transcription complexes.Journal of virology · 2008Article
- An alpha-helical peptidomimetic inhibitor of the HIV-1 Rev-RRE interaction.Journal of the American Chemical Society · 2006Article
- Evolvability of the mode of peptide binding by an RNA.RNA (New York, N.Y.) · 2005Article
- Selection of TAR RNA-binding chameleon peptides by using a retroviral replication system.Journal of virology · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The arginine-rich motif provides a versatile framework for RNA recognition in which few amino acids other than arginine are needed to mediate specific binding. Using a mammalian screening system based on transcriptional activation by HIV Tat, we identified novel arginine-rich peptides from combinatorial libraries that bind tightly to the Rev response element of HIV. Remarkably, a single glutamine, but not asparagine, within a stretch of polyarginine can mediate high-affinity binding. These results, together with the structure of a Rev peptide-Rev response element complex, suggest that the carboxamide groups of glutamine or asparagine are well-suited to hydrogen bond to G-A base pairs and begin to establish an RNA recognition code for the arginine-rich motif. The screening approach may provide a relatively general method for screening expression libraries in mammalian cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.