ArticleProceedings of the National Academy of Sciences of the United States of America1998
Brefeldin A-inhibited guanine nucleotide-exchange activity of Sec7 domain from yeast Sec7 with yeast and mammalian ADP ribosylation factors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 95 citations in OpenAlex.
- BsSYT1 is required for growth, conidiogenesis, stress response and virulence of Bipolaris sorokiniana.World journal of microbiology & biotechnology · 2026Article
- Endocytosis and trans-Golgi Network in Yeast.Sub-cellular biochemistry · 2026Review
- Disordered hinge regions of the AP-3 adaptor complex promote vesicle budding from the late Golgi in yeast.Journal of cell science · 2024Article
- Osh-dependent and -independent Regulation of PI4P Levels During Polarized Growth ofMolecular biology of the cell · 2023Article
- The development of resistance to an inhibitor of a cellular protein reveals a critical interaction between the enterovirus protein 2C and a small GTPase Arf1.PLoS pathogens · 2023Article
- The yeast guanine nucleotide exchange factor Sec7 is a bottleneck in spatial protein quality control and detoxifies neurological disease proteins.Scientific reports · 2023Article
- Common Markers and Small Molecule Inhibitors in Golgi Studies.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Sec71 separates Golgi stacks inJournal of cell science · 2020Article
- Article
- AtTRAPPC11/ROG2: A Role for TRAPPs in Maintenance of the PlantThe Plant cell · 2019Article
- Rab5-mediated endosome formation is regulated at theCommunications biology · 2019Article
- Functional exploration of co-expression networks identifies a nexus for modulating protein and citric acid titres inFungal biology and biotechnology · 2019Article
- NtGNL1a ARF-GEF acts in endocytosis in tobacco cells.BMC plant biology · 2015Article
- Biochemical methods for studying kinetic regulation of Arf1 activation by Sec7.Methods in cell biology · 2015Article
- Contact of cis-Golgi with ER exit sites executes cargo capture and delivery from the ER.Nature communications · 2014Article
- Filamin A mediated Big2 dependent endocytosis: From apical abscission to periventricular heterotopia.Tissue barriers · 2014Article
- Review
- Haploinsufficiency of the Sec7 guanine nucleotide exchange factor gea1 impairs septation in fission yeast.PloS one · 2013Article
- Periventricular Heterotopia: Shuttling of Proteins through Vesicles and Actin in Cortical Development and Disease.Scientifica · 2012Review
- Phosphatidylserine is polarized and required for proper Cdc42 localization and for development of cell polarity.Nature cell biology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Saccharomyces cerevisiae Sec7 protein (ySec7p), which is an important component of the yeast secretory pathway, contains a sequence of approximately 200 amino acids referred to as a Sec7 domain. Similar Sec7 domain sequences have been recognized in several guanine nucleotide-exchange proteins (GEPs) for ADP ribosylation factors (ARFs). ARFs are approximately 20-kDa GTPases that regulate intracellular vesicular membrane trafficking and activate phospholipase D. GEPs activate ARFs by catalyzing the replacement of bound GDP with GTP. We, therefore, undertook to determine whether a Sec7 domain itself could catalyze nucleotide exchange on ARF and found that it exhibited brefeldin A (BFA)-inhibitable ARF GEP activity. BFA is known to inhibit ARF GEP activity in Golgi membranes, thereby causing reversible apparent dissolution of the Golgi complex in many cells. The His6-tagged Sec7 domain from ySec7p (rySec7d) synthesized in Escherichia coli enhanced binding of guanosine 5'-[gamma-[35S]thio]triphosphate by recombinant yeast ARF1 (ryARF1) and ryARF2 but not by ryARF3. The effects of rySec7d on ryARF2 were inhibited by BFA in a concentration-dependent manner but not by inactive analogues of BFA (B-17, B-27, and B-36). rySec7d also promoted BFA-sensitive guanosine 5'-[gamma-thio]triphosphate binding by nonmyristoylated recombinant human ARF1 (rhARF1), rhARF5, and rhARF6, although the effect on rhARF6 was very small. These results are consistent with the conclusion that the yeast Sec7 domain itself contains the elements necessary for ARF GEP activity and its inhibition by BFA.
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