ArticlePlant physiology1998
Actin-bundling protein isolated from pollen tubes of lily. Biochemical and immunocytochemical characterization
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 102 citations in OpenAlex.
- Control of the Actin Cytoskeleton Within Apical and Subapical Regions of Pollen Tubes.Frontiers in cell and developmental biology · 2020Review
- Actin Bundles in The Pollen Tube.International journal of molecular sciences · 2018Review
- A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines.Nature communications · 2018Article
- Article
- Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast.Nature communications · 2016Article
- De novo sequencing and analysis of the lily pollen transcriptome: an open access data source for an orphan plant species.Plant molecular biology · 2015Article
- Organization and regulation of the actin cytoskeleton in the pollen tube.Frontiers in plant science · 2014Review
- Interaction between Calcium and Actin in Guard Cell and Pollen Signaling Networks.Plants (Basel, Switzerland) · 2013Review
- Arabidopsis villins promote actin turnover at pollen tube tips and facilitate the construction of actin collars.The Plant cell · 2013Article
- MAP18 regulates the direction of pollen tube growth in Arabidopsis by modulating F-actin organization.The Plant cell · 2013Article
- FIMBRIN1 is involved in lily pollen tube growth by stabilizing the actin fringe.The Plant cell · 2012Article
- Characterization of the role of calcium in regulating the microtubule-destabilizing activity of MDP25.Plant signaling & behavior · 2012Article
- Plant actin-binding protein SCAB1 is dimeric actin cross-linker with atypical pleckstrin homology domain.The Journal of biological chemistry · 2012Article
- Bundling actin filaments from membranes: some novel players.Frontiers in plant science · 2012Article
- Proteins implicated in mediating self-incompatibility-induced alterations to the actin cytoskeleton of Papaver pollen.Annals of botany · 2011Review
- Arabidopsis VILLIN5, an actin filament bundling and severing protein, is necessary for normal pollen tube growth.The Plant cell · 2010Article
- Arabidopsis VILLIN1 and VILLIN3 have overlapping and distinct activities in actin bundle formation and turnover.The Plant cell · 2010Article
- Actin filament organization and polarity in pollen tubes revealed by myosin II subfragment 1 decoration.Planta · 2008Article
- An actin-binding protein, LlLIM1, mediates calcium and hydrogen regulation of actin dynamics in pollen tubes.Plant physiology · 2008Article
- ACTIN BINDING PROTEIN 29 from Lilium pollen plays an important role in dynamic actin remodeling.The Plant cell · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 135-kD actin-bundling protein was purified from pollen tubes of lily (Lilium longiflorum) using its affinity to F-actin. From a crude extract of the pollen tubes, this protein was coprecipitated with exogenously added F-actin and then dissociated from F-actin by treating it with high-ionic-strength solution. The protein was further purified sequentially by chromatography on a hydroxylapatite column, a gel-filtration column, and a diethylaminoethyl-cellulose ion-exchange column. In the present study, this protein is tentatively referred to as P-135-ABP (Plant 135-kD Actin-Bundling Protein). By the elution position from a gel-filtration column, we estimated the native molecular mass of purified P-135-ABP to be 260 kD, indicating that it existed in a dimeric form under physiological conditions. This protein bound to and bundled F-actin prepared from chicken breast muscle in a Ca2+-independent manner. The binding of 135-P-ABP to actin was saturated at an approximate stoichiometry of 26 actin monomers to 1 dimer of P-135-ABP. By transmission electron microscopy of thin sections, we observed cross-bridges between F-actins with a longitudinal periodicity of 31 nm. Immunofluorescence microscopy using rhodamine-phalloidin and antibodies against the 135-kD polypeptide showed that P-135-ABP was colocalized with bundles of actin filaments in lily pollen tubes, leading us to conclude that it is the factor responsible for bundling the filaments.
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Registered trials
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