ArticlePlant physiology1998
Intracellular pH in arbuscular mycorrhizal fungi. A symbiotic physiological marker
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Mycelial dynamics in arbuscular mycorrhizal fungi.The New phytologist · 2026Review
- Cellular anatomy of arbuscular mycorrhizal fungi.Current biology : CB · 2025Review
- The AMSlide for noninvasive time-lapse imaging of arbuscular mycorrhizal symbiosis.Journal of microscopy · 2025Article
- Efficient Bioimaging with Diaminodicyanoquinodimethanes: Selective Imaging of Epidermal and Stomatal Cells and Insight into the Molecular Level Interactions.Scientific reports · 2017Article
- pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita.Mycorrhiza · 2015Article
- Live-Cell imaging and measurement of intracellular pH in filamentous fungi using a genetically encoded ratiometric probe.Eukaryotic cell · 2009Article
- pH signature for the responses of arbuscular mycorrhizal fungi to external stimuli.Plant signaling & behavior · 2008Article
- GR24, a synthetic analog of strigolactones, stimulates the mitosis and growth of the arbuscular mycorrhizal fungus Gigaspora rosea by boosting its energy metabolism.Plant physiology · 2008Article
- Root factors induce mitochondrial-related gene expression and fungal respiration during the developmental switch from asymbiosis to presymbiosis in the arbuscular mycorrhizal fungus Gigaspora rosea.Plant physiology · 2003Article
- Uranium uptake and translocation by the arbuscular mycorrhizal fungus, Glomus intraradices, under root-organ culture conditions.The New phytologist · 2002Article
- Growing pollen tubes possess a constitutive alkaline band in the clear zone and a growth-dependent acidic tip.The Journal of cell biology · 1999Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method was developed to perform real-time analysis of cytosolic pH of arbuscular mycorrhizal fungi in culture using dye and ratiometric measurements (490/450 nm excitations). The study was mainly performed using photometric analysis, although some data were confirmed using image analysis. The use of nigericin allowed an in vivo calibration. Experimental parameters such as loading time and concentration of the dye were determined so that pH measurements could be made for a steady-state period on viable cells. A characteristic pH profile was observed along hyphae. For Gigaspora margarita, the pH of the tip (0-2 &mgr;m) was typically 6.7, increased sharply to 7.0 behind this region (9.5 &mgr;m), and decreased over the next 250 &mgr;m to a constant value of 6.6. A similar pattern was obtained for Glomus intraradices. The pH profile of G. margarita germ tubes was higher when cultured in the presence of carrot (Daucus carota) hairy roots (nonmycorrhizal). Similarly, extraradical hyphae of G. intraradices had a higher apical pH than the germ tubes. The use of a paper layer to prevent the mycorrhizal roots from being in direct contact with the medium selected hyphae with an even higher cytosolic pH. Results suggest that this method could be useful as a bioassay for studying signal perception and/or H+ cotransport of nutrients by arbuscular mycorrhizal hyphae.
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Registered trials
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