ArticleBiophysical journal1998
Two-dimensional tracking of ncd motility by back focal plane interferometry.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
63 citing papers in PubMed, 235 citations in OpenAlex.
- Goblet cells mechanically breach the epithelial barrier in gut homeostasis.Nature communications · 2026Article
- Attonewton Force Resolution Measurements with Silicon Nanospheres at the Thermal Noise Limit in Ambient-Temperature Liquids.Nano letters · 2025Article
- Kinesin-14 HSET and KlpA are non-processive microtubule motors with load-dependent power strokes.Nature communications · 2024Article
- Cell mediated remodeling of stiffness matched collagen and fibrin scaffolds.Scientific reports · 2022Article
- Flagellar rotational features of an optically confined bacterium at high frequency and temporal resolution reveal the microorganism's response to changes in the fluid environment.European biophysics journal : EBJ · 2022Article
- Patterned photocrosslinking to establish stiffness anisotropies in fibrous 3D hydrogels.Acta biomaterialia · 2022Article
- Particle Classification through the Analysis of the Forward Scattered Signal in Optical Tweezers.Sensors (Basel, Switzerland) · 2021Article
- Optical tweezers in single-molecule biophysics.Nature reviews. Methods primers · 2021Article
- Single-Molecule Studies of Protein Folding with Optical Tweezers.Annual review of biochemistry · 2020Review
- Structured Back Focal Plane Interferometry (SBFPI).Scientific reports · 2019Article
- Back-focal-plane interferometric detection of nanoparticles in spatially confined microfluidic channels.The Review of scientific instruments · 2019Article
- New Detector Sensitivity Calibration and the Calculation of the Interaction Force between Particles Using an Optical Tweezer.Micromachines · 2018Article
- Objective-lens-free Fiber-based Position Detection with Nanometer Resolution in a Fiber Optical Trapping System.Scientific reports · 2017Article
- Direct measurement of cortical force generation and polarization in a living parasite.Molecular biology of the cell · 2017Article
- Achiral symmetry breaking and positive Gaussian modulus lead to scalloped colloidal membranes.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- In situ calibration of position detection in an optical trap for active microrheology in viscous materials.Optics express · 2017Article
- Biophysical properties of human β-cardiac myosin with converter mutations that cause hypertrophic cardiomyopathy.Science advances · 2017Article
- Article
- Contractility parameters of human β-cardiac myosin with the hypertrophic cardiomyopathy mutation R403Q show loss of motor function.Science advances · 2015Article
- Origins of Escherichia coli growth rate and cell shape changes at high external osmolality.Biophysical journal · 2014Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A technique for detecting the displacement of micron-sized optically trapped probes using far-field interference is introduced, theoretically explained, and used to study the motility of the ncd motor protein. Bead motions in the focal plane relative to the optical trap were detected by measuring laser intensity shifts in the back-focal plane of the microscope condenser by projection on a quadrant diode. This detection method is two-dimensional, largely independent of the position of the trap in the field of view and has approximately 10-micros time resolution. The high resolution makes it possible to apply spectral analysis to measure dynamic parameters such as local viscosity and attachment compliance. A simple quantitative theory for back-focal-plane detection was derived that shows that the laser intensity shifts are caused primarily by a far-field interference effect. The theory predicts the detector response to bead displacement, without adjustable parameters, with good accuracy. To demonstrate the potential of the method, the ATP-dependent motility of ncd, a kinesin-related motor protein, was observed with an in vitro bead assay. A fusion protein consisting of truncated ncd (amino acids 195-685) fused with glutathione-S-transferase was adsorbed to silica beads, and the axial and lateral motions of the beads along the microtubule surface were observed with high spatial and temporal resolution. The average axial velocity of the ncd-coated beads was 230 +/- 30 nm/s (average +/- SD). Spectral analysis of bead motion showed the increase in viscous drag near the surface; we also found that any elastic constraints of the moving motors are much smaller than the constraints due to binding in the presence of the nonhydrolyzable nucleotide adenylylimidodiphosphate.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.