ArticleBiophysical journal1998
Regulation of exocytotic fusion by cell inflation.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 88 citations in OpenAlex.
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- Prestin kinetics and corresponding frequency dependence augment during early development of the outer hair cell within the mouse organ of Corti.Scientific reports · 2019Article
- Calcium-dependent, non-apoptotic, large plasma membrane bleb formation in physiologically stimulated mast cells and basophils.Journal of extracellular vesicles · 2019Article
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- The Actomyosin Cytoskeleton Drives Micron-Scale Membrane Remodeling In Vivo Via the Generation of Mechanical Forces to Balance Membrane Tension Gradients.BioEssays : news and reviews in molecular, cellular and developmental biology · 2018Review
- Characterization of ion channels and OJournal of neurophysiology · 2017Article
- Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis.Journal of visualized experiments : JoVE · 2017Article
- A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells.eLife · 2015Article
- Real-time imaging of microparticles and living cells with CMOS nanocapacitor arrays.Nature nanotechnology · 2015Article
- The Arabidopsis Vacuolar Sorting Receptor1 is required for osmotic stress-induced abscisic acid biosynthesis.Plant physiology · 2015Article
- Mechanisms of gain control by voltage-gated channels in intrinsically-firing neurons.PloS one · 2015Article
- Mechanosensitive channels: feeling tension in a world under pressure.Frontiers in plant science · 2014Review
- Tonotopic relationships reveal the charge density varies along the lateral wall of outer hair cells.Biophysical journal · 2012Article
- Exocytosis in the frog amphibian papilla.Journal of the Association for Research in Otolaryngology : JARO · 2012Article
- Selective cell-surface labeling of the molecular motor protein prestin.Biochemical and biophysical research communications · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have inflated patch-clamped mast cells by 3.8 +/- 1.6 times their volume by applying a hydrostatic pressure of 5-15 cm H2O to the interior of the patch pipette. Inflation did not cause changes in the cell membrane conductance and caused only a small reversible change in the cell membrane capacitance (36 +/- 5 fF/cm H2O). The specific cell membrane capacitance of inflated cells was found to be 0.5 microF/cm2. High-resolution capacitance recordings showed that inflation reduced the frequency of exocytotic fusion events by approximately 70-fold, with the remaining fusion events showing an unusual time course. Shortly after the pressure was returned to 0 cm H2O, mast cells regained their normal size and appearance and degranulated completely, even after remaining inflated for up to 60 min. We interpret these observations as an indication that inflated mast cells reversibly disassemble the structures that regulate exocytotic fusion. Upon returning to its normal size, the cell cytosol reassembles the fusion pore scaffolds and allows exocytosis to proceed, suggesting that exocytotic fusion does not require soluble proteins. Reassembly of the fusion pore can be prevented by inflating the cells with solutions containing the protease pronase, which completely blocked exocytosis. We also interpret these results as evidence that the activity of the fusion pore is sensitive to the tension of the plasma membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.