ArticleBiophysical journal1998
Analysis of various sequence-specific triplexes by electron and atomic force microscopies.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Nucleic Acid-Based Field-Effect Transistor Biosensors.Biosensors · 2026Review
- A New Strategy to Investigate RNA:DNA Triplex Using Atomic Force Microscopy.International journal of molecular sciences · 2024Article
- A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices.Micromachines · 2022Review
- Atomic force microscopy-A tool for structural and translational DNA research.APL bioengineering · 2021Review
- The dynamics of forming a triplex in an artificial telomere inferred by DNA mechanics.Nucleic acids research · 2019Article
- Preparation of DNA and nucleoprotein samples for AFM imaging.Micron (Oxford, England : 1993) · 2011Review
- Article
- The triple helix: 50 years later, the outcome.Nucleic acids research · 2008Review
- Gapped DNA and cyclization of short DNA fragments.Biophysical journal · 2005Article
- Inducing and modulating anisotropic DNA bends by pseudocomplementary peptide nucleic acids.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Padlock oligonucleotides as a tool for labeling superhelical DNA.Nucleic acids research · 2002Article
- Atomic force microscopy of the submolecular architecture of hydrated ocular mucins.Biophysical journal · 1999Article
- Visualization of trp repressor and its complexes with DNA by atomic force microscopy.Biophysical journal · 1998Article
- Short unligated sticky ends enable the observation of circularised DNA by atomic force and electron microscopies.Nucleic acids research · 1998Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequence-specific interactions of 20-mer G,A-containing triple helix-forming oligonucleotides (TFOs) and bis-PNAs (peptide nucleic acids) with double-stranded DNA was visualized by electron (EM) and atomic force (AFM) microscopies. Triplexes formed by biotinylated TFOs are easily detected by both EM and AFM in which streptavidin is a marker. AFM images of the unlabeled triplex within a long plasmid DNA show a approximately 0.4-nm height increment of the double helix within the target site position. TFOs conjugated to a 74-nt-long oligonucleotide forming a 33-bp-long hairpin form extremely stable triplexes with the target site that are readily imaged by both EM and AFM as protruding DNA. The short duplex protrudes in a perpendicular direction relative to the double helix axis, either in the plane of the support or out of it. In the latter case, the apparent height of the protrusion is approximately 1.5 nm, when that of the triplex site is increased by 0.3-0.4 nm. Triplex formation by bis-PNA, in which two decamers of PNA are connected via a flexible linker, causes deformations of the double helix at the target site, which is readily detected as kinks by both EM and AFM. Moreover, AFM shows that these kinks are often accompanied by an increase in the DNA apparent height of approximately 35%. This work shows the first direct visualization of sequence-specific interaction of TFOs and PNAs, with their target sequences within long plasmid DNAs, through the measurements of the apparent height of the DNA double helix by AFM.
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