ArticleThe Journal of cell biology1998
The Ig superfamily cell adhesion molecule, apCAM, mediates growth cone steering by substrate-cytoskeletal coupling.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
102 citing papers in PubMed, 204 citations in OpenAlex.
- Actomyosin dynamics in detached cells: linking clutch model to cell migration and cytokinesis.BMC molecular and cell biology · 2025Article
- A simple active fluid model unites cytokinesis, cell crawling, and axonal outgrowth.bioRxiv : the preprint server for biology · 2024Article
- A modified motor-clutch model reveals that neuronal growth cones respond faster to soft substrates.Molecular biology of the cell · 2024Article
- Measuring Retrograde Actin Flow in Neuronal Growth Cones.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A simple active fluid model unites cytokinesis, cell crawling, and axonal outgrowth.Frontiers in cell and developmental biology · 2024Article
- Adhesion-clutch between DCC and netrin-1 mediates netrin-1-induced axonal haptotaxis.Frontiers in molecular neuroscience · 2024Article
- With the Permission of Microtubules: An Updated Overview on Microtubule Function During Axon Pathfinding.Frontiers in molecular neuroscience · 2021Review
- Manipulation of Axonal Outgrowth via Exogenous Low Forces.International journal of molecular sciences · 2020Review
- Regulation of axon growth by myosin II-dependent mechanocatalysis of cofilin activity.The Journal of cell biology · 2019Article
- Article
- Lamellipodium is a myosin-independent mechanosensor.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- An Integrated Cytoskeletal Model of Neurite Outgrowth.Frontiers in cellular neuroscience · 2018Review
- Neurite elongation is highly correlated with bulk forward translocation of microtubules.Scientific reports · 2017Article
- Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation.Molecular biology of the cell · 2017Article
- Astrocytes in Migration.Neurochemical research · 2017Review
- Directional Transport of a Bead Bound to Lamellipodial Surface Is Driven by Actin Polymerization.BioMed research international · 2017Article
- Cell adhesion and invasion mechanisms that guide developing axons.Current opinion in neurobiology · 2016Review
- Guidance of Axons by Local Coupling of Retrograde Flow to Point Contact Adhesions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Nerve growth factor stimulates axon outgrowth through negative regulation of growth cone actomyosin restraint of microtubule advance.Molecular biology of the cell · 2016Article
- Increase in Growth Cone Size Correlates with Decrease in Neurite Growth Rate.Neural plasticity · 2016Article
42 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Dynamic cytoskeletal rearrangements are involved in neuronal growth cone motility and guidance. To investigate how cell surface receptors translate guidance cue recognition into these cytoskeletal changes, we developed a novel in vitro assay where beads, coated with antibodies to the immunoglobulin superfamily cell adhesion molecule apCAM or with purified native apCAM, replaced cellular substrates. These beads associated with retrograde F-actin flow, but in contrast to previous studies, were then physically restrained with a microneedle to simulate interactions with noncompliant cellular substrates. After a latency period of approximately 10 min, we observed an abrupt increase in bead-restraining tension accompanied by direct extension of the microtubule-rich central domain toward sites of apCAM bead binding. Most importantly, we found that retrograde F-actin flow was attenuated only after restraining tension had increased and only in the bead interaction axis where preferential microtubule extension occurred. These cytoskeletal and structural changes are very similar to those reported for growth cone interactions with physiological targets. Immunolocalization using an antibody against the cytoplasmic domain of apCAM revealed accumulation of the transmembrane isoform of apCAM around bead-binding sites. Our results provide direct evidence for a mechanical continuum from apCAM bead substrates through the peripheral domain to the central cytoplasmic domain. By modulating functional linkage to the underlying actin cytoskeleton, cell surface receptors such as apCAM appear to enable the application of tensioning forces to extracellular substrates, providing a mechanism for transducing retrograde flow into guided growth cone movement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.