ArticleThe Journal of cell biology1998
Apical plasma membrane proteins and endolyn-78 travel through a subapical compartment in polarized WIF-B hepatocytes.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 113 citations in OpenAlex.
- CD164 is a host factor for lymphocytic choriomeningitis virus entry.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Link between Intrahepatic Cholestasis and Genetic Variations in Intracellular Trafficking Regulators.Biology · 2021Review
- Rab17 regulates apical delivery of hepatic transcytotic vesicles.Molecular biology of the cell · 2018Article
- Manganese transport and toxicity in polarized WIF-B hepatocytes.American journal of physiology. Gastrointestinal and liver physiology · 2018Article
- The GTP-bound and Sumoylated Form of the rab17 Small Molecular Weight GTPase Selectively Binds Syntaxin 2 in Polarized Hepatic WIF-B Cells.The Journal of biological chemistry · 2016Article
- Myosin Vb mediates Cu+ export in polarized hepatocytes.Journal of cell science · 2016Article
- Copper directs ATP7B to the apical domain of hepatic cells via basolateral endosomes.Traffic (Copenhagen, Denmark) · 2014Article
- The unique polarity phenotype of hepatocytes.Experimental cell research · 2014Review
- Importance of endocytic pathways in liver function and disease.Comprehensive Physiology · 2014Review
- Distinct phenotype of a Wilson disease mutation reveals a novel trafficking determinant in the copper transporter ATP7B.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Hepatocyte polarity.Comprehensive Physiology · 2013Review
- Apical targeting and endocytosis of the sialomucin endolyn are essential for establishment of zebrafish pronephric kidney function.Journal of cell science · 2012Article
- Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism.Molecular biology of the cell · 2012Article
- Cellular function and molecular structure of ecto-nucleotidases.Purinergic signalling · 2012Review
- Mutation conferring apical-targeting motif on AE1 exchanger causes autosomal dominant distal RTA.Journal of the American Society of Nephrology : JASN · 2012Article
- CD13 regulates dendritic cell cross-presentation and T cell responses by inhibiting receptor-mediated antigen uptake.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Article
- Analysis of polarized membrane traffic in hepatocytes and hepatic cell lines.Current protocols in cell biology · 2012Article
- Multiple biosynthetic trafficking routes for apically secreted proteins in MDCK cells.Traffic (Copenhagen, Denmark) · 2012Article
- Lipid droplet accumulation and impaired fat efflux in polarized hepatic cells: consequences of ethanol metabolism.International journal of hepatology · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We studied basolateral-to-apical transcytosis of three classes of apical plasma membrane (PM) proteins in polarized hepatic WIF-B cells and then compared it to the endocytic trafficking of basolaterally recycling membrane proteins. We used antibodies to label the basolateral cohort of proteins at the surface of living cells and then followed their trafficking at 37 degreesC by indirect immunofluorescence. The apical PM proteins aminopeptidase N, 5'nucleotidase, and the polymeric IgA receptor were efficiently transcytosed. Delivery to the apical PM was confirmed by microinjection of secondary antibodies into the bile canalicular-like space and by EM studies. Before acquiring their apical steady-state distribution, the trafficked antibodies accumulated in a subapical compartment, which had a unique tubulovesicular appearance by EM. In contrast, antibodies to the receptors for asialoglycoproteins and mannose-6-phosphate or to the lysosomal membrane protein, lgp120, distributed to endosomes or lysosomes, respectively, without accumulating in the subapical area. However, the route taken by the endosomal/lysosomal protein endolyn-78 partially resembled the transcytotic pathway, since anti-endolyn-78 antibodies were found in a subapical compartment before delivery to lysosomes. Our results suggest that in WIF-B cells, transcytotic molecules pass through a subapical compartment that functions as a second sorting site for a subset of basolaterally endocytosed membrane proteins reaching this compartment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.