ArticleThe Biochemical journal1998
Nucleolar protein p120 contains an arginine-rich domain that binds to ribosomal RNA.
Article in The Biochemical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 52 citations in OpenAlex.
- Aberrant NSUN1 activity connects mLife science alliance · 2026Article
- Nucleolar protein NOP2/NSUN1 suppresses HIV-1 transcription and promotes viral latency by competing with Tat for TAR binding and methylation.PLoS pathogens · 2020Article
- HIV-1 Replication Benefits from the RNA Epitranscriptomic Code.Journal of molecular biology · 2019Review
- Nop2 is required for mammalian preimplantation development.Molecular reproduction and development · 2016Article
- Posttranscriptional methylation of transfer and ribosomal RNA in stress response pathways, cell differentiation, and cancer.Current opinion in oncology · 2016Review
- Eukaryotic rRNA Modification by Yeast 5-Methylcytosine-Methyltransferases and Human Proliferation-Associated Antigen p120.PloS one · 2015Article
- Role of RNA methyltransferases in tissue renewal and pathology.Current opinion in cell biology · 2014Review
- Characterizing 5-methylcytosine in the mammalian epitranscriptome.Genome biology · 2013Review
- Identification of tobacco proteins associated with the stem-loop 1 RNAs of Potato virus X.Molecules and cells · 2012Article
- RNA content in the nucleolus alters p53 acetylation via MYBBP1A.The EMBO journal · 2011Article
- 5-methylcytosine in RNA: detection, enzymatic formation and biological functions.Nucleic acids research · 2010Review
- Clusters of basic amino acids contribute to RNA binding and nucleolar localization of ribosomal protein L22.PloS one · 2009Article
- Bop1 is a mouse WD40 repeat nucleolar protein involved in 28S and 5. 8S RRNA processing and 60S ribosome biogenesis.Molecular and cellular biology · 2000Article
- Myc induces the nucleolin and BN51 genes: possible implications in ribosome biogenesis.Nucleic acids research · 2000Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human proliferation-associated protein p120 has previously been shown to localize to the nucleolus, and several functional domains of p120 have been elucidated. By using a nitrocellulose filter binding assay and a Northwestern blotting procedure this study shows that recombinant p120 binds to an rRNA fragment in vitro with a dissociation constant of 4 nM. The specific RNA-binding region of p120 (residues 1-57) was identified with glutathione S-transferase-fused p120 deletion constructs and Northwestern blotting procedures. This RNA-binding region of p120, which includes the nucleolar localization signal of p120, is similar to the arginine-rich RNA-binding regions found in other RNA-binding proteins such as HIV Rev and Tat. Experiments in vivo with HeLa cell nucleolar extracts showed that p120 was associated with the 60-80S pre-ribosomal particles. This association is disrupted by treatment with either RNase A or buffer of high ionic strength. These results suggest that p120 might be involved in rRNA/ribosome maturation, consistent with the role of the yeast homologue Nop2p in rRNA biogenesis.
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