ArticleGlycoconjugate journal1998
Multiplex RT-PCR method for the analysis of the expression of human sialyltransferases: application to breast cancer cells.
Article in Glycoconjugate journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- When a negative (charge) is not a positive: sialylation and its role in cancer mechanics and progression.Frontiers in oncology · 2024Review
- High-MannoseCancers · 2022Article
- Heterogeneity in 2,6-Linked Sialic Acids Potentiates Invasion of Breast Cancer Epithelia.ACS central science · 2021Article
- Regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene expression during differentiation of human osteoblastic MG-63 cells.Glycoconjugate journal · 2020Article
- Nuclear repartitioning of galectin-1 by an extracellular glycan switch regulates mammary morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Nutrient-deprived cancer cells preferentially use sialic acid to maintain cell surface glycosylation.Biomaterials · 2015Article
- α2,6-linked sialic acids on N-glycans modulate the adhesion of hepatocarcinoma cells to lymph nodes.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation.The Journal of biological chemistry · 2014Article
- Preferential lectin binding of cancer cells upon sialic acid treatment under nutrient deprivation.Applied biochemistry and biotechnology · 2013Article
- Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.PloS one · 2013Article
- Altered expression of sialylated glycoproteins in breast cancer using hydrazide chemistry and mass spectrometry.Molecular & cellular proteomics : MCP · 2012Article
- Emerging role of alpha2,6-sialic acid as a negative regulator of galectin binding and function.The Journal of biological chemistry · 2011Review
- Transcriptional regulation of the human ST6GAL2 gene in cerebral cortex and neuronal cells.Glycoconjugate journal · 2010Article
- Tumor cell migration and invasion are regulated by expression of variant integrin glycoforms.Experimental cell research · 2008Article
- Influence of TNFalpha on the sialylation of mucins produced by a transformed cell line MM-39 derived from human tracheal gland cells.Glycoconjugate journal · 2001Article
- Sialylation of E-cadherin does not change the spontaneous or ET-18-OMe-mediated aggregation of MCF-7 human breast cancer cells.Clinical & experimental metastasis · 1999Article
- Permanent exposure of mucin-secreting HT-29 cells to benzyl-N-acetyl-alpha-D-galactosaminide induces abnormal O-glycosylation of mucins and inhibits constitutive and stimulated MUC5AC secretion.The Biochemical journal · 1998Article
- GalNAc-alpha-O-benzyl inhibits NeuAcalpha2-3 glycosylation and blocks the intracellular transport of apical glycoproteins and mucus in differentiated HT-29 cells.The Journal of cell biology · 1998Article
- Sialyltransferases in cancer.Glycoconjugate journalReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many cases of human cancer, the appearance of hypersialylated glycan structures is related to a precise stage of the disease; this may depend on altered regulation of one or more sialyltransferases genes. Since several distinct sialyltransferase enzymes arising from different unique genes transfer sialic acid residues in the same linkage onto the same acceptor, it is impossible to precisely determine which enzyme is involved in the observed phenotype based on enzymatic assays. We have developed a very sensitive and highly reproducible multiplex reverse transcriptase-polymerase chain reaction technique in order to monitor the expression of four human sialyltransferases genes ST6Gal I, ST3Gal I, ST3Gal III and ST3Gal IV in small cell samples. Multiplex PCR amplification using specific primers for each sialyltransferase and detection of amplification products by polyacrylamide gel electrophoresis is a method that is fast and easy to handle and has proven to be useful for establishing sialyltransferase patterns of expression in breast immortalized cell line HBL100 as well as in breast cancer cell lines MCF-7/6, MCF-7/AZ and MDA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.