ArticleMolecular biology of the cell1998
Homology-dependent gene silencing in Paramecium.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 167 citations in OpenAlex.
- Subtle-seq reveals frequent ageing-associated chromosome fragmentation on nucleosome core DNA.Nucleic acids research · 2026Article
- Application of RNA interference and protein localization to investigate housekeeping and developmentally regulated genes in the emerging model protozoan Paramecium caudatum.Communications biology · 2024Article
- Micromanipulation in Paramecium: From non-mendelian inheritance to the outlook for versatile micromachines.The Journal of eukaryotic microbiology · 2022Review
- Not all Is SET for Methylation: Evolution of Eukaryotic Protein Methyltransferases.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Programmed Chromosome Deletion in the CiliateG3 (Bethesda, Md.) · 2019Article
- C11orf70 Mutations Disrupting the Intraflagellar Transport-Dependent Assembly of Multiple Axonemal Dyneins Cause Primary Ciliary Dyskinesia.American journal of human genetics · 2018Article
- Environmental Temperature Controls Accumulation of Transacting siRNAs Involved in Heterochromatin Formation.Genes · 2018Article
- Two Sets of Piwi Proteins Are Involved in Distinct sRNA Pathways Leading to Elimination of Germline-Specific DNA.Cell reports · 2017Article
- Two sets of RNAi components are required for heterochromatin formation in trans triggered by truncated transgenes.Nucleic acids research · 2016Article
- DNA interference: DNA-induced gene silencing in the appendicularian Oikopleura dioica.Proceedings. Biological sciences · 2015Article
- Primary and secondary siRNA synthesis triggered by RNAs from food bacteria in the ciliate Paramecium tetraurelia.Nucleic acids research · 2015Article
- Programmed Genome Rearrangements in the Ciliate Oxytricha.Microbiology spectrum · 2014Review
- Genome-wide analysis of genetic and epigenetic control of programmed DNA deletion.Nucleic acids research · 2014Article
- A forward genetic screen reveals essential and non-essential RNAi factors in Paramecium tetraurelia.Nucleic acids research · 2014Article
- Pdsg1 and Pdsg2, novel proteins involved in developmental genome remodelling in Paramecium.PloS one · 2014Article
- Transgene-mediated co-suppression of DNA topoisomerase-1 gene in Caenorhabditis elegans.International journal of biochemistry and molecular biology · 2014Article
- Epigenetics of ciliates.Cold Spring Harbor perspectives in biology · 2013Review
- Outer dynein arm light chain 1 is essential for controlling the ciliary response to cyclic AMP in Paramecium tetraurelia.Eukaryotic cell · 2012Article
- Functional specialization of Piwi proteins in Paramecium tetraurelia from post-transcriptional gene silencing to genome remodelling.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microinjection at high copy number of plasmids containing only the coding region of a gene into the Paramecium somatic macronucleus led to a marked reduction in the expression of the corresponding endogenous gene(s). The silencing effect, which is stably maintained throughout vegetative growth, has been observed for all Paramecium genes examined so far: a single-copy gene (ND7), as well as members of multigene families (centrin genes and trichocyst matrix protein genes) in which all closely related paralogous genes appeared to be affected. This phenomenon may be related to posttranscriptional gene silencing in transgenic plants and quelling in Neurospora and allows the efficient creation of specific mutant phenotypes thus providing a potentially powerful tool to study gene function in Paramecium. For the two multigene families that encode proteins that coassemble to build up complex subcellular structures the analysis presented herein provides the first experimental evidence that the members of these gene families are not functionally redundant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.