ArticleMolecular biology of the cell1998
Arp2/3 complex from Acanthamoeba binds profilin and cross-links actin filaments.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 92 citations in OpenAlex.
- Profilin and Its Isoforms: Unravelling the Functional Diversity Across Life Forms.Current protein & peptide science · 2026Review
- Branched actin cortices reconstituted in vesicles sense membrane curvature.Biophysical journal · 2023Article
- Actomyosin-Driven Division of a Synthetic Cell.ACS synthetic biology · 2022Review
- A model of actin-driven endocytosis explains differences of endocytic motility in budding and fission yeast.Molecular biology of the cell · 2022Article
- Transcriptional loss of domestication-driven cytoskeletal GhPRF1 gene causes defective floral and fiber development in cotton (Gossypium).Plant molecular biology · 2021Article
- Syndecan-4 in Tumor Cell Motility.Cancers · 2021Review
- Profilin Isoforms in Health and Disease - All the Same but Different.Frontiers in cell and developmental biology · 2021Review
- Actin assembly produces sufficient forces for endocytosis in yeast.Molecular biology of the cell · 2019Article
- Proteomic approach for understanding milder neurotoxicity of Carfilzomib against Bortezomib.Scientific reports · 2018Article
- Reconsidering an active role for G-actin in cytoskeletal regulation.Journal of cell science · 2018Review
- Profilin Interaction with Actin Filament Barbed End Controls Dynamic Instability, Capping, Branching, and Motility.Developmental cell · 2016Article
- Control of polarized assembly of actin filaments in cell motility.Cellular and molecular life sciences : CMLS · 2015Review
- Profilin-1 serves as a gatekeeper for actin assembly by Arp2/3-dependent and -independent pathways.Developmental cell · 2015Article
- Rac deletion in osteoclasts causes severe osteopetrosis.Journal of cell science · 2011Article
- Arp2/3 complex activity in filopodia of spreading cells.BMC cell biology · 2008Article
- Mutations in actin-related proteins 2 and 3 affect cell shape development in Arabidopsis.The Plant cell · 2003Article
- Relating interactions between neurofilaments to the structure of axonal neurofilament distributions through polymer brush models.Biophysical journal · 2002Article
- Actin-based motility of intracellular microbial pathogens.Microbiology and molecular biology reviews : MMBR · 2001Review
- Profilin plays a role in cell elongation, cell shape maintenance, and flowering in Arabidopsis.Plant physiology · 2000Article
- Profilin enhances Cdc42-induced nucleation of actin polymerization.The Journal of cell biology · 2000Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The Arp2/3 complex was first purified from Acanthamoeba castellanii by profilin affinity chromatography. The mechanism of interaction with profilin was unknown but was hypothesized to be mediated by either Arp2 or Arp3. Here we show that the Arp2 subunit of the complex can be chemically cross-linked to the actin-binding site of profilin. By analytical ultracentrifugation, rhodamine-labeled profilin binds Arp2/3 complex with a Kd of 7 microM, an affinity intermediate between the low affinity of profilin for barbed ends of actin filaments and its high affinity for actin monomers. These data suggest the barbed end of Arp2 is exposed, but Arp2 and Arp3 are not packed together in the complex exactly like two actin monomers in a filament. Arp2/3 complex also cross-links actin filaments into small bundles and isotropic networks, which are mechanically stiffer than solutions of actin filaments alone. Arp2/3 complex is concentrated at the leading edge of motile Acanthamoeba, and its localization is distinct from that of alpha-actinin, another filament cross-linking protein. Based on localization and actin filament nucleation and cross-linking activities, we propose a role for Arp2/3 in determining the structure of the actin filament network at the leading edge of motile cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.