ArticleMolecular biology of the cell1998
Spc98p directs the yeast gamma-tubulin complex into the nucleus and is subject to cell cycle-dependent phosphorylation on the nuclear side of the spindle pole body.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 85 citations in OpenAlex.
- MiR-4763-3p accelerates lipopolysaccharide-induced cardiomyocyte apoptosis and inflammatory response by targeting IL10RA.Cytotechnology · 2024Article
- Keratin 17 regulates nuclear morphology and chromatin organization.Journal of cell science · 2020Article
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- Tubgcp3 Is Required for Retinal Progenitor Cell Proliferation During Zebrafish Development.Frontiers in molecular neuroscience · 2019Article
- Novel phosphorylation states of the yeast spindle pole body.Biology open · 2018Article
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- Synergistic role of fission yeast Alp16GCP6 and Mzt1MOZART1 in γ-tubulin complex recruitment to mitotic spindle pole bodies and spindle assembly.Molecular biology of the cell · 2016Article
- Interaction of CK1δ with γTuSC ensures proper microtubule assembly and spindle positioning.Molecular biology of the cell · 2015Article
- Cell-cycle dependent phosphorylation of yeast pericentrin regulates γ-TuSC-mediated microtubule nucleation.eLife · 2014Article
- An extended γ-tubulin ring functions as a stable platform in microtubule nucleation.The Journal of cell biology · 2012Article
- Mitotic spindle form and function.Genetics · 2012Article
- Nucleocytoplasmic shuttling of cytoskeletal proteins: molecular mechanism and biological significance.International journal of cell biology · 2012Article
- The MPS1 family of protein kinases.Annual review of biochemistry · 2012Review
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- Structural mutants of the spindle pole body cause distinct alteration of cytoplasmic microtubules and nuclear dynamics in multinucleated hyphae.Molecular biology of the cell · 2010Article
- Budding yeast centrosome duplication requires stabilization of Spc29 via Mps1-mediated phosphorylation.The Journal of biological chemistry · 2009Article
- Nuclear export receptor Xpo1/Crm1 is physically and functionally linked to the spindle pole body in budding yeast.Molecular and cellular biology · 2008Article
- Nucleocytoplasmic shuttling of the Rpb4p and Rpb7p subunits of Saccharomyces cerevisiae RNA polymerase II by two pathways.Eukaryotic cell · 2006Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the yeast Saccharomyces cerevisiae, microtubules are organized by the spindle pole body (SPB), which is embedded in the nuclear envelope. Microtubule organization requires the gamma-tubulin complex containing the gamma-tubulin Tub4p, Spc98p, and Spc97p. The Tub4p complex is associated with cytoplasmic and nuclear substructures of the SPB, which organize the cytoplasmic and nuclear microtubules. Here we present evidence that the Tub4p complex assembles in the cytoplasm and then either binds to the cytoplasmic side of the SPB or is imported into the nucleus followed by binding to the nuclear side of the SPB. Nuclear import of the Tub4p complex is mediated by the essential nuclear localization sequence of Spc98p. Our studies also indicate that Spc98p in the Tub4p complex is phosphorylated at the nuclear, but not at the cytoplasmic, side of the SPB. This phosphorylation is cell cycle dependent and occurs after SPB duplication and nucleation of microtubules by the new SPB and therefore may have a role in mitotic spindle function. In addition, activation of the mitotic checkpoint stimulates Spc98p phosphorylation. The kinase Mps1p, which functions in SPB duplication and mitotic checkpoint control, seems to be involved in Spc98p phosphorylation. Our results also suggest that the nuclear and cytoplasmic Tub4p complexes are regulated differently.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.