ArticleMolecular biology of the cell1998
Assembly of lampbrush chromosomes from sperm chromatin.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Lampbrush chromosomes of Danio rerio.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Characterization of axolotl lampbrush chromosomes by fluorescence in situ hybridization and immunostaining.Experimental cell research · 2021Article
- Cold shock induces novel nuclear bodies in Xenopus oocytes.Experimental cell research · 2021Review
- Determinants of Chromosome Architecture: Insulator Pairing in cis and in trans.PLoS genetics · 2016Article
- The Egg and the Nucleus: A Battle for Supremacy.Rambam Maimonides medical journal · 2015Article
- Hierarchical molecular events driven by oocyte-specific factors lead to rapid and extensive reprogramming.Molecular cell · 2014Article
- Induction of human lampbrush chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Chromomeres revisited.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Are lampbrush chromosomes unique to meiotic cells?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Stable intronic sequence RNA (sisRNA), a new class of noncoding RNA from the oocyte nucleus of Xenopus tropicalis.Genes & development · 2012Article
- Widespread transcription in an amphibian oocyte relates to its reprogramming activity on transplanted somatic nuclei.Stem cells and development · 2012Article
- Lampbrush chromosomes enable study of cohesin dynamics.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- The tripartite motif of nuclear factor 7 is required for its association with transcriptional units.Molecular and cellular biology · 2007Article
- Localized co-transcriptional recruitment of the multifunctional RNA-binding protein CELF1 by lampbrush chromosome transcription units.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Article
- Cohesion proteins are present in centromere protein bodies associated with avian lampbrush chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2005Article
- Subnuclear localization and Cajal body targeting of transcription elongation factor TFIIS in amphibian oocytes.Molecular biology of the cell · 2003Article
- Distribution of XCAP-E and XCAP-D2 in the Xenopus oocyte nucleus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Article
- Lampbrush chromosomes and associated bodies: new insights into principles of nuclear structure and function.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2002Review
- Nuclear accumulation of S-adenosylhomocysteine hydrolase in transcriptionally active cells during development of Xenopus laevis.Molecular biology of the cell · 1999Article
- The stem-loop binding protein (SLBP1) is present in coiled bodies of the Xenopus germinal vesicle.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
We have examined the behavior of demembranated sperm heads when injected into the germinal vesicle (GV) of amphibian oocytes. Xenopus sperm heads injected into Xenopus GVs swelled immediately and within hours began to stain with an antibody against RNA polymerase II (Pol II). Over time each sperm head became a loose mass of chromosome-like threads, which by 24-48 h resolved into individually recognizable lampbrush chromosomes (LBCs). Although LBCs derived from sperm are unreplicated single chromatids, their morphology and immunofluorescent staining properties were strikingly similar to those of the endogenous lampbrush bivalents. They displayed typical transcriptionally active loops extending from an axis of condensed chromomeres, as well as locus-specific "landmarks. " Experiments with [3H]GTP and actinomycin D demonstrated that transcription was not necessary for the initial swelling of the sperm heads and acquisition of Pol II but was required for maintenance of the lampbrush loops. Splicing was not required at any stage during formation of sperm LBCs. When Xenopus sperm heads were injected into GVs of the newt Notophthalmus, the resulting sperm LBCs displayed very long loops with pronounced Pol II axes, like those of the endogenous newt LBCs; as expected, they stained with antibodies against newt-specific proteins. Other heterologous injections, including sperm heads of the frog Rana pipiens and the zebrafish Danio rerio in Xenopus GVs, confirm that LBCs can be derived from taxonomically distant organisms. The GV system should help identify both cis- and trans-acting factors needed to convert condensed chromatin into transcriptionally active LBCs. It may also be useful in producing cytologically analyzable chromosomes from organisms whose oocytes do not go through a typical lampbrush phase or cannot be manipulated by current techniques.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.