ArticleMolecular and cellular biology1998
Human matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogaster.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 95 citations in OpenAlex.
- Dynamics of nuclear matrix attachment regions during 5BMC genomics · 2022Article
- Identification of a potent MAR element from the human genome and assessment of its activity in stably transfected CHO cells.Journal of cellular and molecular medicine · 2018Article
- Article
- Sensitivity of Allelic Divergence to Genomic Position: Lessons from the Drosophila tan Gene.G3 (Bethesda, Md.) · 2016Article
- Suppressor of hairy-wing, modifier of mdg4 and centrosomal protein of 190 gene orthologues of the gypsy insulator complex in the malaria mosquito, Anopheles stephensi.Insect molecular biology · 2016Article
- Exogenous gypsy insulator sequences modulate transgene expression in the malaria vector mosquito, Anopheles stephensi.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- New properties of Drosophila scs and scs' insulators.PloS one · 2013Article
- Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators.Plant cell reports · 2012Review
- Analysis of the enhancer-blocking function of the TBS element from Petunia hybrida in transgenic Arabidopsis thaliana and Nicotiana tabacum.Plant cell reports · 2011Article
- Development of a BAC vector for integration-independent and tight regulation of transgenes in rodents via the Tet system.Transgenic research · 2011Article
- Current advances in retroviral gene therapy.Current gene therapy · 2011Review
- A BEAF dependent chromatin domain boundary separates myoglianin and eyeless genes of Drosophila melanogaster.Nucleic acids research · 2011Article
- Enhancer-promoter interference and its prevention in transgenic plants.Plant cell reports · 2011Review
- Mutation of a barrier insulator in the human ankyrin-1 gene is associated with hereditary spherocytosis.The Journal of clinical investigation · 2010Article
- Article
- Drosophila mini-white model system: new insights into positive position effects and the role of transcriptional terminators and gypsy insulator in transgene shielding.Nucleic acids research · 2010Article
- Vertebrate Protein CTCF and its Multiple Roles in a Large-Scale Regulation of Genome Activity.Current genomics · 2009Article
- Functional characterization of a tobacco matrix attachment region-mediated enhancement of transgene expression.Transgenic research · 2009Article
- An insulator with barrier-element activity promotes alpha-spectrin gene expression in erythroid cells.Blood · 2009Article
- Red flag on the white reporter: a versatile insulator abuts the white gene in Drosophila and is omnipresent in mini-white constructs.Nucleic acids research · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Germ line transformation of white- Drosophila embryos with P-element vectors containing white expression cassettes results in flies with different eye color phenotypes due to position effects at the sites of transgene insertion. These position effects can be cured by specific DNA elements, such as the Drosophila scs and scs' elements, that have insulator activity in vivo. We have used this system to determine whether human matrix attachment regions (MARs) can function as insulator elements in vivo. Two different human MARs, from the apolipoprotein B and alpha1-antitrypsin loci, insulated white transgene expression from position effects in Drosophila melanogaster. Both elements reduced variability in transgene expression without enhancing levels of white gene expression. In contrast, expression of white transgenes containing human DNA segments without matrix-binding activity was highly variable in Drosophila transformants. These data indicate that human MARs can function as insulator elements in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.