ArticleMolecular and cellular biology1998
Characterization of BCE-1, a transcriptional enhancer regulated by prolactin and extracellular matrix and modulated by the state of histone acetylation.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 103 citations in OpenAlex.
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- Orientation and repositioning of chromosomes correlate with cell geometry-dependent gene expression.Molecular biology of the cell · 2017Article
- Organoids: A historical perspective of thinking in three dimensions.The Journal of cell biology · 2017Review
- Dynamic reciprocity between cells and their microenvironment in reproduction.Biology of reproduction · 2015Review
- Constructing three-dimensional models to study mammary gland branching morphogenesis and functional differentiation.Journal of mammary gland biology and neoplasia · 2012Review
- Growth of lung cancer cells in three-dimensional microenvironments reveals key features of tumor malignancy.Integrative biology : quantitative biosciences from nano to macro · 2012Article
- Depletion of nuclear actin is a key mediator of quiescence in epithelial cells.Journal of cell science · 2011Article
- Raf-induced MMP9 disrupts tissue architecture of human breast cells in three-dimensional culture and is necessary for tumor growth in vivo.Genes & development · 2010Article
- Laminin regulates PI3K basal localization and activation to sustain STAT5 activation.Cell cycle (Georgetown, Tex.) · 2010Article
- The contribution of adhesion signaling to lactogenesis.Journal of cell communication and signaling · 2010Article
- Connective Tissue Growth Factor (CTGF/CCN2) enhances lactogenic differentiation of mammary epithelial cells via integrin-mediated cell adhesion.BMC cell biology · 2010Article
- The epigenetic landscape of mammary gland development and functional differentiation.Journal of mammary gland biology and neoplasia · 2010Review
- Gene expression in the third dimension: the ECM-nucleus connection.Journal of mammary gland biology and neoplasia · 2010Review
- Tissue architecture and function: dynamic reciprocity via extra- and intra-cellular matrices.Cancer metastasis reviews · 2009Review
- Extracellular matrix control of mammary gland morphogenesis and tumorigenesis: insights from imaging.Histochemistry and cell biology · 2008Review
- Architecture Is the Message: The role of extracellular matrix and 3-D structure in tissue-specific gene expression and breast cancer.The Pezcoller Foundation journal : news from the Pezcoller Foundation world · 2007Article
- Cell shape regulates global histone acetylation in human mammary epithelial cells.Experimental cell research · 2007Article
- Extracellular matrix-regulated gene expression requires cooperation of SWI/SNF and transcription factors.The Journal of biological chemistry · 2007Article
- Epigenetic reversion of breast carcinoma phenotype is accompanied by changes in DNA sequestration as measured by AluI restriction enzyme.The American journal of pathology · 2007Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously described a 160-bp enhancer (BCE-1) in the bovine beta-casein gene that is activated in the presence of prolactin and extracellular matrix (ECM). Here we report the characterization of the enhancer by deletion and site-directed mutagenesis, electrophoretic mobility shift analysis, and in vivo footprinting. Two essential regions were identified by analysis of mutant constructions: one binds C/EBP-beta and the other binds MGF/STAT5 and an as-yet-unidentified binding protein. However, no qualitative or quantitative differences in the binding of these proteins were observed in electrophoretic mobility shift analysis using nuclear extracts derived from cells cultured in the presence or absence of ECM with or without prolactin, indicating that prolactin- and ECM-induced transcription was not dependent on the availability of these factors in the functional cell lines employed. An in vivo footprinting analysis of the factors bound to nuclear chromatin in the presence or absence of ECM and/or prolactin found no differences in the binding of C/EBP-beta but did not provide definitive results for the other factors. Neither ECM nor prolactin activated BCE-1 in transient transfections, suggesting that the chromosomal structure of the integrated template may be required for ECM-induced transcription. Further evidence is that treatment of cells with inhibitors of histone deacetylase was sufficient to induce transcription of integrated BCE-1 in the absence of ECM. Together, these results suggest that the ECM induces a complex interaction between the enhancer-bound transcription factors, the basal transcriptional machinery, and a chromosomally integrated template responsive to the acetylation state of the histones.
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