ArticleMolecular and cellular biology1998
Functions of the N- and C-terminal domains of human RAP74 in transcriptional initiation, elongation, and recycling of RNA polymerase II.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- CTDP1 and RPB7 stabilize Pol II and permit reinitiation.Nature communications · 2025Article
- Gdown1 Associates Efficiently with RNA Polymerase II after Promoter Clearance and Displaces TFIIF during Transcript Elongation.PloS one · 2016Article
- Functional interactions of the RNA polymerase II-interacting proteins Gdown1 and TFIIF.The Journal of biological chemistry · 2014Article
- The RNA polymerase II preinitiation complex. Through what pathway is the complex assembled?Transcription · 2014Review
- Promoter clearance by RNA polymerase II.Biochimica et biophysica acta · 2013Review
- The functions of TFIIF during initiation and transcript elongation are differentially affected by phosphorylation by casein kinase 2.The Journal of biological chemistry · 2011Article
- Structural characterization of human general transcription factor TFIIF in solution.Protein science : a publication of the Protein Society · 2008Article
- Purification and identification of proteins that bind to the hereditary persistence of fetal hemoglobin -198 mutation in the gamma-globin gene promoter.The Journal of biological chemistry · 2007Article
- Amino acid substitutions in yeast TFIIF confer upstream shifts in transcription initiation and altered interaction with RNA polymerase II.Molecular and cellular biology · 2004Article
- A nonconserved surface of the TFIIB zinc ribbon domain plays a direct role in RNA polymerase II recruitment.Molecular and cellular biology · 2004Article
- FCP1, a phosphatase specific for the heptapeptide repeat of the largest subunit of RNA polymerase II, stimulates transcription elongation.Molecular and cellular biology · 2002Article
- Crystal structure of the C-terminal domain of the RAP74 subunit of human transcription factor IIF.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Cyclin D1 suppresses retinoblastoma protein-mediated inhibition of TAFII250 kinase activity.Oncogene · 2000Article
- Mechanism of promoter melting by the xeroderma pigmentosum complementation group B helicase of transcription factor IIH revealed by protein-DNA photo-cross-linking.Molecular and cellular biology · 2000Article
- Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- The zinc ribbon domains of the general transcription factors TFIIB and Brf: conserved functional surfaces but different roles in transcription initiation.Genes & development · 2000Article
- The RAP74 subunit of human transcription factor IIF has similar roles in initiation and elongation.Molecular and cellular biology · 1999Article
- A region within the RAP74 subunit of human transcription factor IIF is critical for initiation but dispensable for complex assembly.Molecular and cellular biology · 1999Article
- DNA bending and wrapping around RNA polymerase: a "revolutionary" model describing transcriptional mechanisms.Microbiology and molecular biology reviews : MMBR · 1999Review
- DNA wrapping in transcription initiation by RNA polymerase II.Biochemistry and cell biology = Biochimie et biologie cellulaire · 1999Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription factor IIF (TFIIF) cooperates with RNA polymerase II (pol II) during multiple stages of the transcription cycle including preinitiation complex assembly, initiation, elongation, and possibly termination and recycling. Human TFIIF appears to be an alpha2beta2 heterotetramer of RNA polymerase II-associating protein 74- and 30-kDa subunits (RAP74 and RAP30). From inspection of its 517-amino-acid (aa) sequence, the RAP74 subunit appears to comprise separate N- and C-terminal domains connected by a flexible loop. In this study, we present functional data that strongly support this model for RAP74 architecture and further show that the N- and C-terminal domains and the central loop of RAP74 have distinct roles during separate phases of the transcription cycle. The N-terminal domain of RAP74 (minimally aa 1 to 172) is sufficient to deliver pol II into a complex formed on the adenovirus major late promoter with the TATA-binding protein, TFIIB, and RAP30. A more complete N-terminal domain fragment (aa 1 to 217) strongly stimulates both accurate initiation and elongation by pol II. The region of RAP74 between aa 172 and 205 and a subregion between aa 170 and 178 are critical for both accurate initiation and elongation, and mutations in these regions have similar effects on initiation and elongation. Based on these observations, RAP74 appears to have similar functions in initiation and elongation. The central region and the C-terminal domain of RAP74 do not contribute strongly to single-round accurate initiation or elongation stimulation but do stimulate multiple-round transcription in an extract system.
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