ArticleMolecular and cellular biology1998
A heat-sensitive Arabidopsis thaliana kinase substitutes for human p70s6k function in vivo.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 63 citations in OpenAlex.
- Phosphorylation of a reinitiation supporting protein, RISP, determines its function in translation reinitiation.Nucleic acids research · 2021Article
- The Role of Ribosomal Protein S6 Kinases in Plant Homeostasis.Frontiers in molecular biosciences · 2021Review
- Heat-induced inhibition of phosphorylation of the stress-protective transcription factor DREB2A promotes thermotolerance ofThe Journal of biological chemistry · 2019Article
- Recent Discoveries on the Role of TOR (Target of Rapamycin) Signaling in Translation in Plants.Plant physiology · 2018Review
- Root Cell-Specific Regulators of Phosphate-Dependent Growth.Plant physiology · 2017Article
- The Arabidopsis TOR Kinase Specifically Regulates the Expression of Nuclear Genes Coding for Plastidic Ribosomal Proteins and the Phosphorylation of the Cytosolic Ribosomal Protein S6.Frontiers in plant science · 2016Article
- Spatial Regulation of Root Growth: Placing the Plant TOR Pathway in a Developmental Perspective.International journal of molecular sciences · 2015Review
- Target of rapamycin signaling regulates metabolism, growth, and life span in Arabidopsis.The Plant cell · 2012Article
- Diversity, classification and function of the plant protein kinase superfamily.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Article
- Dynamic Light Regulation of Translation Status in Arabidopsis thaliana.Frontiers in plant science · 2012Article
- Monitoring molecular-specific pharmacodynamics of rapamycin in vivo with inducible Gal4->Fluc transgenic reporter mice.Molecular cancer therapeutics · 2010Article
- Arabidopsis S6 kinase mutants display chromosome instability and altered RBR1-E2F pathway activity.The EMBO journal · 2010Article
- The two Arabidopsis RPS6 genes, encoding for cytoplasmic ribosomal proteins S6, are functionally equivalent.Plant molecular biology · 2010Article
- Hsp70 associates with Rictor and is required for mTORC2 formation and activity.Biochemical and biophysical research communications · 2008Article
- Transcriptional responses of Arabidopsis thaliana plants to As (V) stress.BMC plant biology · 2008Article
- Stress and mTORture signaling.Oncogene · 2006Review
- Arabidopsis TARGET OF RAPAMYCIN interacts with RAPTOR, which regulates the activity of S6 kinase in response to osmotic stress signals.The Plant cell · 2006Article
- Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression.Plant physiology · 2005Article
- High heterogeneity within the ribosomal proteins of the Arabidopsis thaliana 80S ribosome.Plant molecular biology · 2005Article
- Proteomic characterization of evolutionarily conserved and variable proteins of Arabidopsis cytosolic ribosomes.Plant physiology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammalian cells, mitogen-induced phosphorylation of ribosomal protein S6 by p70s6k has been implicated in the selective translational upregulation of 5'TOP mRNAs. We demonstrate here that the homologous Arabidopsis thaliana protein, AtS6k2, ectopically expressed in human 293 cells or isolated from plant cells, phosphorylates specifically mammalian and plant S6 at 25 degrees C but not at 37 degrees C. When Arabidopsis suspension culture cells are shifted from 25 to 37 degrees C, the kinase becomes rapidly inactivated, consistent with the observation that heat shock abrogates S6 phosphorylation in plants. Treatment with potato acid phosphatase reduced the specific activity of immunoprecipitated AtS6k2 threefold, an effect which was blocked in the presence of 4-nitrophenyl phosphate. In quiescent mammalian cells, AtS6k2 is activated by serum stimulation, a response which is abolished by the fungal metabolite wortmannin but is resistant to rapamycin. Treatment of mammalian cells with rapamycin abolishes in vivo S6 phosphorylation by p70s6k; however, ectopic expression of AtS6k2 rescues the rapamycin block. Collectively, the data demonstrate that AtS6k2 is the functional plant homolog of mammalian p70s6k and identify a new signalling pathway in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.