ArticleMolecular and cellular biology1998
Analysis of the interaction of the novel RNA polymerase II (pol II) subunit hsRPB4 with its partner hsRPB7 and with pol II.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 55 citations in OpenAlex.
- The RNA Pol II sub-complex hsRpb4/7 is required for viability of multiple human cell lines.Protein & cell · 2012Article
- Human GTPases associate with RNA polymerase II to mediate its nuclear import.Molecular and cellular biology · 2011Article
- mRNA imprinting: Additional level in the regulation of gene expression.Cellular logistics · 2011Article
- NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation.Genes & development · 2009Article
- Genomewide recruitment analysis of Rpb4, a subunit of polymerase II in Saccharomyces cerevisiae, reveals its involvement in transcription elongation.Eukaryotic cell · 2008Article
- Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors.Methods in molecular biology (Clifton, N.J.) · 2005Article
- Domainal organization of the lower eukaryotic homologs of the yeast RNA polymerase II core subunit Rpb7 reflects functional conservation.Nucleic acids research · 2004Article
- Identification of the RNA polymerase II subunit hsRPB7 as a novel target of the von Hippel-Lindau protein.The EMBO journal · 2003Article
- Architecture of initiation-competent 12-subunit RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- An Rpb4/Rpb7-like complex in yeast RNA polymerase III contains the orthologue of mammalian CGRP-RCP.Molecular and cellular biology · 2003Article
- Two different Drosophila ADA2 homologues are present in distinct GCN5 histone acetyltransferase-containing complexes.Molecular and cellular biology · 2003Article
- The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Rpb4 and Rpb9 mediate subpathways of transcription-coupled DNA repair in Saccharomyces cerevisiae.The EMBO journal · 2002Article
- Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.Molecular and cellular biology · 2002Article
- Inhibitors of Ras/Raf-1 interaction identified by two-hybrid screening revert Ras-dependent transformation phenotypes in human cancer cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II.Molecular and cellular biology · 2002Article
- Article
- Suprabasal overexpression of the hsRPB7 gene in psoriatic epidermis as identified by a reverse transcriptase-polymerase chain reaction differential display model comparing psoriasis plaque tissue with peritonsillar mucosa.The American journal of pathology · 2001Article
- A human RNA polymerase II subunit is encoded by a recently generated multigene family.BMC molecular biology · 2001Article
- Archaeal RNA polymerase subunits F and P are bona fide homologs of eukaryotic RPB4 and RPB12.Nucleic acids research · 2000Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Under conditions of environmental stress, prokaryotes and lower eukaryotes such as the yeast Saccharomyces cerevisiae selectively utilize particular subunits of RNA polymerase II (pol II) to alter transcription to patterns favoring survival. In S. cerevisiae, a complex of two such subunits, RPB4 and RPB7, preferentially associates with pol II during stationary phase; of these two subunits, RPB4 is specifically required for survival under nonoptimal growth conditions. Previously, we have shown that RPB7 possesses an evolutionarily conserved human homolog, hsRPB7, which was capable of partially interacting with RPB4 and the yeast transcriptional apparatus. Using this as a probe in a two-hybrid screen, we have now established that hsRPB4 is also conserved in higher eukaryotes. In contrast to hsRPB7, hsRPB4 has diverged so that it no longer interacts with yeast RPB7, although it partially complements rpb4- phenotypes in yeast. However, hsRPB4 associates strongly and specifically with hsRPB7 when expressed in yeast or in mammalian cells and copurifies with intact pol II. hsRPB4 expression in humans parallels that of hsRPB7, supporting the idea that the two proteins may possess associated functions. Structure-function studies of hsRPB4-hsRPB7 are used to establish the interaction interface between the two proteins. This identification completes the set of human homologs for RNA pol II subunits defined in yeast and should provide the basis for subsequent structural and functional characterization of the pol II holoenzyme.
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