ArticleMolecular and cellular biology1998
A differential screen for ligand-regulated genes: identification of HoxA10 as a target of vitamin D3 induction in myeloid leukemic cells.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 83 citations in OpenAlex.
- MicroRNA profiles were altered in neonatal piglet mammary glands following postnatal infant formula feeding.The Journal of nutritional biochemistry · 2020Article
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- HOXA10 controls osteoblastogenesis by directly activating bone regulatory and phenotypic genes.Molecular and cellular biology · 2007Article
- Brap2 functions as a cytoplasmic retention protein for p21 during monocyte differentiation.Molecular and cellular biology · 2004Article
- Role of homeobox genes in normal mammary gland development and breast tumorigenesis.Journal of mammary gland biology and neoplasia · 2003Review
- The ontogeny of 25-hydroxyvitamin D(3) 1alpha-hydroxylase expression in human placenta and decidua.The American journal of pathology · 2002Article
- Normal myelopoiesis but abnormal T lymphocyte responses in vitamin D receptor knockout mice.The Journal of clinical investigation · 2002Article
- p21 is a transcriptional target of HOXA10 in differentiating myelomonocytic cells.Genes & development · 2000Article
- Homeobox genes in mammary gland development and neoplasia.Breast cancer research : BCR · 2000Review
- Extracellular matrix signaling: integration of form and function in normal and malignant cells.Current opinion in cell biology · 1998Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
1,25-Dihydroxyvitamin D3 [1,25(OH)2D3], the hormonal ligand for vitamin D3, is a potent inducer of myeloid-leukemic-cell differentiation. Such cells differentiate exclusively into monocytes/macrophages in response to this ligand. Since 1,25(OH)2D3 transduces its hormone signal through the vitamin D3 receptor (VDR), a ligand-modulated transcription factor and member of the nuclear hormone receptor superfamily, we sought to identify direct VDR target genes induced during this differentiation process. To do so, we applied a modified differential screen with a nascent-RNA purification strategy using biases for immediate-early-response genes induced by 1,25(OH)2D3 in the myelomonocytic cell line U937. Using this screen, we had previously identified p21Waf1/Cip1 as a gene transcriptionally induced by 1,25(OH)2D3 and demonstrated that this induction facilitates the differentiation of U937 cells into monocytes/macrophages (24). Here, we describe in detail our differential screen strategy and the identification and isolation of 20 1,25(OH)2D3-inducible genes or unknown cDNAs by means of this screen. One gene newly identified as a target of VDR regulation in myeloid cells is the homeobox HoxA10 gene. HoxA10 protein may act as a general regulator of cell growth, since overexpression of HoxA10 facilitated the differentiation of U937 cells into monocytes/macrophages independent of 1,25(OH)2D3 and acted to strongly inhibit the growth of the breast cancer cell line MCF-7 by arresting these cells in G1.
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