ArticleMolecular and cellular biology1998
Characterization of Stat5a and Stat5b homodimers and heterodimers and their association with the glucocortiocoid receptor in mammary cells.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 162 citations in OpenAlex.
- TCR γδ cell-specific STAT5 gain of function induces a druggable chronic human immune dysregulation.Journal of human immunity · 2026Article
- An RNA-sequencing analysis to determine potential upstream transcriptional regulators of essential amino acid deficiency responses in bovine mammary epithelial cells.BMC genomics · 2026Article
- Lactose on the basolateral side of mammary epithelial cells inhibits milk production concomitantly with signal transducer and activator of transcription 5 inactivation.Cell and tissue research · 2022Article
- Remodeling of ZnMetallomics : integrated biometal science · 2020Article
- Balancing STAT Activity as a Therapeutic Strategy.Cancers · 2019Review
- Superiority of SpiroZin2 Versus FluoZin-3 for monitoring vesicular ZnScientific reports · 2018Article
- Review
- Lineage-Specific and Non-specific Cytokine-Sensing Genes Respond Differentially to the Master Regulator STAT5.Cell reports · 2016Article
- Review
- Distinct roles of STAT3 and STAT5 in the pathogenesis and targeted therapy of breast cancer.Molecular and cellular endocrinology · 2014Review
- Dynamic trafficking of STAT5 depends on an unconventional nuclear localization signal.Journal of cell science · 2013Article
- Signal transducer and activator of transcription 5 as a key signaling pathway in normal mammary gland developmental biology and breast cancer.Breast cancer research : BCR · 2011Review
- Minireview: Glucocorticoids in autoimmunity: unexpected targets and mechanisms.Molecular endocrinology (Baltimore, Md.) · 2011Review
- Prolactin inhibits BCL6 expression in breast cancer through a Stat5a-dependent mechanism.Cancer research · 2010Article
- Lactogenic hormonal induction of long distance interactions between beta-casein gene regulatory elements.The Journal of biological chemistry · 2009Article
- Role of c-Myb during prolactin-induced signal transducer and activator of transcription 5a signaling in breast cancer cells.Endocrinology · 2009Article
- STAT5 mediates antiapoptotic effects of methylprednisolone on oligodendrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Lipogenesis impaired in periparturient rats exposed to altered gravity is independent of prolactin and glucocorticoid secretion.European journal of applied physiology · 2008Article
- The role of glucocorticoids in secretory activation and milk secretion, a historical perspective.Journal of mammary gland biology and neoplasia · 2007Review
- Involvement of the ubiquitous Oct-1 transcription factor in hormonal induction of beta-casein gene expression.The Biochemical journal · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lactogenic hormones, i.e., prolactin and glucocorticoids, act in concert to stimulate transcription factors responsible for hormone-dependent milk protein gene expression. In the mammary gland, prolactin activates Stat5a and Stat5b and glucocorticoids activate the glucocorticoid receptor (GR). Immunoprecipitation experiments revealed that in mammary cells, Stat5a, Stat5b, and the GR are physically associated in vivo. The association is not dependent on lactogenic hormone treatment and is evident at all stages of mammary gland development. Immunodepletion experiments indicated that a fraction of GR and Stat5 proteins are not associated, suggesting that there are different intracellular pools of these proteins. Lactogenic hormone treatment of HC11 mammary cells resulted in tyrosine phosphorylation of Stat5a and Stat5b, dimerization, and rapid nuclear translocation of both Stat5 proteins. Following hormone treatment, Stat5a-Stat5b heterodimers were detected by their coimmunoprecipitation. In addition, immunodepletion experiments followed by gel shift analyses revealed the presence of active Stat5a and Stat5b homodimers. In mammary cells, Stat5b homodimers are less abundant than Stat5a homodimers. Although the GR does not bind the Stat5 DNA binding site directly, it could be detected with the Stat5-DNA complex. These results suggest that glucocorticoids affect milk protein gene expression via association of the GR with Stat5. Thus, there is a functional coupling between Stat-dependent and nuclear hormone receptor-dependent gene transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.