ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1998
A reluctant gating mode of glycine receptor channels determines the time course of inhibitory miniature synaptic events in zebrafish hindbrain neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 77 citations in OpenAlex.
- Glycine receptor and release site organization impacts the kinetics of glycinergic synapse currents.Biophysical journal · 2025Article
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- Alfaxalone Causes Reduction of Glycinergic IPSCs, but Not Glutamatergic EPSCs, and Activates a Depolarizing Current in Rat Hypoglossal Motor Neurons.Frontiers in cellular neuroscience · 2019Article
- Structure-Function Analysis of the GlyR α2 Subunit Autism Mutation p.R323L Reveals a Gain-of-Function.Frontiers in molecular neuroscience · 2017Article
- Investigating the Mechanism by Which Gain-of-function Mutations to the α1 Glycine Receptor Cause Hyperekplexia.The Journal of biological chemistry · 2016Article
- The Free Zinc Concentration in the Synaptic Cleft of Artificial Glycinergic Synapses Rises to At least 1 μM.Frontiers in molecular neuroscience · 2016Article
- Target-specific IPSC kinetics promote temporal processing in auditory parallel pathways.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Frequency-Dependent Cannabinoid Receptor-Independent Modulation of Glycine Receptors by Endocannabinoid 2-AG.Frontiers in molecular neuroscience · 2011Article
- What single-channel analysis tells us of the activation mechanism of ligand-gated channels: the case of the glycine receptor.The Journal of physiology · 2010Review
- Single-channel kinetic analysis for activation and desensitization of homomeric 5-HT(3)A receptors.Biophysical journal · 2009Article
- Properties of glycine receptors underlying synaptic currents in presynaptic axon terminals of rod bipolar cells in the rat retina.The Journal of physiology · 2009Article
- Number and locations of agonist binding sites required to activate homomeric Cys-loop receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Slow glycinergic transmission mediated by transmitter pooling.Nature neuroscience · 2009Article
- Glycine Receptors Caught between Genome and Proteome - Functional Implications of RNA Editing and Splicing.Frontiers in molecular neuroscience · 2009Article
- Embryonic motor activity and implications for regulating motoneuron axonal pathfinding in zebrafish.The European journal of neuroscience · 2008Article
- Extrasynaptic and postsynaptic receptors in glycinergic and GABAergic neurotransmission: a division of labor?Frontiers in molecular neuroscience · 2008Article
- AMPA receptors associated with zebrafish Mauthner cells switch subunits during development.The Journal of physiology · 2007Article
- Spontaneous IPSCs and glycine receptors with slow kinetics in wide-field amacrine cells in the mature rat retina.The Journal of physiology · 2007Article
- Functional properties of spontaneous IPSCs and glycine receptors in rod amacrine (AII) cells in the rat retina.The Journal of physiology · 2006Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Miniature IPSCs (mIPSCs) recorded in the Mauthner (M)-cell of zebrafish larvae have a broad amplitude distribution that is attributable only partly to the functional heterogeneity of postsynaptic glycine receptors (GlyRs). The role of the kinetic properties of GlyRs in amplitude fluctuation was investigated using fast-flow application techniques on outside-out patches. Short applications of a saturating glycine concentration evoked outside-out currents with a biphasic deactivation phase as observed for mIPSCs, and they were consistent with a rapid clearance of glycine from the synaptic cleft. Patch currents declined slowly during continuous applications of 3 mM glycine, but the biphasic deactivation phase of mIPSCs cannot reflect a desensitization process because paired-pulse desensitization was not observed. The maximum open probability (Po) of GlyRs was close to 0.9 with 3 mM glycine. Analyses of the onset of outside-out currents evoked by 0.1 mM glycine are consistent with the presence of two equivalent binding sites with a Kd of O.3-O.4 mM. Activation and deactivation properties of GlyRs were better described with a kinetic model, including two binding states, a doubly liganded open state, and a reluctant gating mode leading to another open state. The 20-80% rise time of mIPSCs was independent of their amplitude and is identical to that of outside-out currents evoked by the applications of a saturating concentration of glycine (>1 mM). These results support the hypothesis that GlyR kinetics determines the time course of synaptic events at M-cell inhibitory synapses and that large mIPSC amplitude fluctuations are mainly of postsynaptic origin.
Indexed as
Identifiers
9526003PMC6792586W2104004306What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.