ArticleThe EMBO journal1998
Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 76 citations in OpenAlex.
- Identification of RNA binding proteins that mediate a quality control mechanism of splicing.bioRxiv : the preprint server for biology · 2025Article
- Evaluation of novel computational methods to identify RNA-binding protein footprints from structural data.RNA (New York, N.Y.) · 2025Article
- The splicing factor DHX38 enables retinal development through safeguarding genome integrity.iScience · 2023Article
- The splicing factor DHX38/PRP16 is required for ovarian clear cell carcinoma tumorigenesis, as revealed by a CRISPR-Cas9 screen.FEBS open bio · 2022Article
- Article
- Arabidopsis AtPRP17 functions in embryo development by regulating embryonic patterning.Planta · 2021Article
- Confirmation of the Role of DHX38 in the Etiology of Early-Onset Retinitis Pigmentosa.Investigative ophthalmology & visual science · 2018Article
- The Fission Yeast Pre-mRNA-processing Factor 18 (prp18+) Has Intron-specific Splicing Functions with Links to G1-S Cell Cycle Progression.The Journal of biological chemistry · 2016Article
- Complementation of Yeast Genes with Human Genes as an Experimental Platform for Functional Testing of Human Genetic Variants.Genetics · 2015Article
- The Arabidopsis ortholog of the DEAH-box ATPase Prp16 influences auxin-mediated development.Plant signaling & behavior · 2015Article
- Splicing functions and global dependency on fission yeast slu7 reveal diversity in spliceosome assembly.Molecular and cellular biology · 2013Article
- ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex.Genes & development · 2010Article
- Contribution of DEAH-box protein DHX16 in human pre-mRNA splicing.The Biochemical journal · 2010Article
- PRP-17 and the pre-mRNA splicing pathway are preferentially required for the proliferation versus meiotic development decision and germline sex determination in Caenorhabditis elegans.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Article
- PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4.The Journal of biological chemistry · 2008Article
- Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions.Molecular and cellular biology · 2006Article
- A novel SR-related protein is required for the second step of Pre-mRNA splicing.Molecular and cellular biology · 2005Article
- Dependence of pre-mRNA introns on PRP17, a non-essential splicing factor: implications for efficient progression through cell cycle transitions.Nucleic acids research · 2003Article
- Reversible inhibition of the second step of splicing suggests a possible role of zinc in the second step of splicing.Nucleic acids research · 2002Article
- PSF and p54nrb bind a conserved stem in U5 snRNA.RNA (New York, N.Y.) · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pre-mRNA splicing takes place in two catalytic steps. The second step is poorly understood, especially in mammals. In yeast, the splicing factors, Prps 16, 17, 18 and Slu7 function exclusively in step II. Here we report the isolation of cDNAs encoding human Prps 16 and 17 which are 41 and 36% identical to their yeast counterparts. The Prp16 gene is essential in yeast, and we show that a chimeric yeast-human Prp16 protein rescues a yeast Prp16 knockout strain. Immunodepletion of hPrp16 from splicing extracts specifically blocks step II, and the activity can be fully restored with recombinant hPrp16. Moreover, both hPrps 16 and 17 associate with the spliceosome late in the splicing pathway. Mutations at the 3' splice site that specifically block step II do not affect the association of hPrps 16 and 17 with the spliceosome, indicating that these factors may function at a stage of step II prior to recognition of the 3' splice site. Recently, the human homologs of Prp18 and Slu7 were identified. The observation that humans contain homologs of all four known step II proteins in yeast indicates that the mechanism for catalytic step II is highly conserved.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.