ArticlePharmaceutical research1998
Biochemical characterization of a glucocorticoid-induced membrane protein (RM3/1) in human monocytes and its application as model system for ranking glucocorticoid potency.
Article in Pharmaceutical research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 43 citations in OpenAlex.
- Systemic corticosteroid therapy augmentsPostepy dermatologii i alergologii · 2020Article
- Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line.BioMed research international · 2018Article
- The role of tumor necrosis factor-like weak inducer of apoptosis in atherosclerosis via its two different receptors.Experimental and therapeutic medicine · 2017Article
- Association between the haptoglobin and heme oxygenase 1 genetic profiles and soluble CD163 in susceptibility to and severity of human malaria.Infection and immunity · 2012Article
- Evolution of the CD163 family and its relationship to the bovine gamma delta T cell co-receptor WC1.BMC evolutionary biology · 2010Article
- Levels of soluble CD163 and severity of malaria in children in Ghana.Clinical and vaccine immunology : CVI · 2008Article
- Pharmacokinetics and pharmacodynamics of systemically administered glucocorticoids.Clinical pharmacokinetics · 2005Review
- Enhanced anti-inflammatory potency of a nitric oxide-releasing prednisolone derivative in the rat.British journal of pharmacology · 2003Article
- 21-NO-prednisolone is a novel nitric oxide-releasing derivative of prednisolone with enhanced anti-inflammatory properties.British journal of pharmacology · 2000Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeUpon glucocorticoid stimulation, human mononuclear leucocytes express an antigen, RM3/1, which characterizes a subpopulation of human monocytes and macrophages evolving in late phase of inflammation. We investigated biochemical properties of the RM3/1 antigen and correlations between antigen expression and glucocorticoid potency.
methodsBiochemical properties were analyzed after solubilization by immunoaffinity methods and SDS-PAGE.
resultsInduction of the RM3/1 antigen is a glucocorticoid receptor mediated process, in contrast, inflammatory mediators such as LPS or TPA were not able to upregulate RM3/1 expression. After SDS-PAGE, the antigen appeared as a 130 kDa (nonreduced)/150 kDa (reduced) glycoprotein with a 25 kDa N-linked glycoportion. The interdependence between antigen density and glucocorticoid efficacy was assessed by calculation of relative antigen expression induced by dexamethasone, fluticasone propionate, budesonide, triamcinolone acetonide, flunisolide, beclomethasone, prednisolone and triamcinolone. Relative antigen expression was significantly correlated with the relative receptor affinity of the glucocorticoid.
conclusionsWe described biochemical properties of the glucocorticoid-induced protein RM3/1. Though the precise role of the RM3/1 antigen in the antiinflammatory process is not fully understood yet, an useful application of the induced expression could already be demonstrated for pre-clinical screening of glucocorticoid potency.
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