ArticleProceedings of the National Academy of Sciences of the United States of America1998
Proteolytic processing of the Aplysia egg-laying hormone prohormone.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed.
- Two- and three-dimensional neuropeptidomic landscape in the central nervous system of an invertebrate chordate,iScience · 2025Article
- Neuropeptidomics of Genetically Defined Cell Types in Mouse Brain.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Current Challenges and Future Directions in Peptidomics.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Profiling 26,000 Aplysia californica neurons by single cell mass spectrometry reveals neuronal populations with distinct neuropeptide profiles.The Journal of biological chemistry · 2022Article
- ADVANCES IN HIGH-RESOLUTION MALDI MASS SPECTROMETRY FOR NEUROBIOLOGY.Mass spectrometry reviews · 2022Review
- Peptide identifications and false discovery rates using different mass spectrometry platforms.Talanta · 2018Article
- Improved identification and quantitation of mature endogenous peptides in the rodent hypothalamus using a rapid conductive sample heating system.The Analyst · 2017Article
- A neuron-in-capillary platform for facile collection and mass spectrometric characterization of a secreted neuropeptide.Scientific reports · 2016Article
- Nanomanipulation-Coupled Matrix-Assisted Laser Desorption/ Ionization-Direct Organelle Mass Spectrometry: A Technique for the Detailed Analysis of Single Organelles.Journal of the American Society for Mass Spectrometry · 2016Article
- Stimulation and release from neurons via a dual capillary collection device interfaced to mass spectrometry.The Analyst · 2013Article
- Article
- Collection of peptides released from single neurons with particle-embedded monolithic capillaries followed by detection with matrix-assisted laser desorption/ionization mass spectrometry.Analytical chemistry · 2011Article
- Recent advances in single-cell MALDI mass spectrometry imaging and potential clinical impact.Expert review of proteomics · 2011Review
- Profiling metabolites and peptides in single cells.Nature methods · 2011Review
- Direct cellular peptidomics of supraoptic magnocellular and hippocampal neurons in low-density co-cultures.ACS chemical neuroscience · 2010Article
- MALDI mass spectrometric imaging using the stretched sample method to reveal neuropeptide distributions in aplysia nervous tissue.Analytical chemistry · 2009Article
- Comparative distribution of a putative egg-laying hormone in neural and reproductive tissues of four Decapoda crustaceans.Invertebrate neuroscience : IN · 2009Article
- Molecular social interactions: Drosophila melanogaster seminal fluid proteins as a case study.Advances in genetics · 2009Article
- Secondary ion MS imaging to relatively quantify cholesterol in the membranes of individual cells from differentially treated populations.Analytical chemistry · 2007Article
- Profiling signaling peptides in single mammalian cells using mass spectrometry.Analytical chemistry · 2006Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
By using matrix-assisted laser desorption/ionization time-of-flight MS, individual peptidergic neurons from Aplysia are assayed. A semiquantitative method is developed for comparing single-cell profiles by using spectral normalization, and peptides are localized to specific cells by mass spectrometric cell mapping. In addition to all previously identified products of the egg-laying hormone (ELH) gene, other peptides are formed from proteolytic hydrolysis of Leu-Leu residues within ELH and acidic peptide (AP). AP exhibits further processing to yield AP1-20 and AP9-27. These peptides appear to be colocalized in vesicles with ELH, transported to specific neuronal targets, and released in a Ca2+-dependent manner. A differential peptide distribution is observed at a specific target cell, and a low-frequency variation of AP, [Thr21]AP, is detected in a single animal.
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