ArticleProceedings of the National Academy of Sciences of the United States of America1998
Probing the genetic population structure of Trypanosoma cruzi with polymorphic microsatellites.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 176 citations in OpenAlex.
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- Wide reference databases for typing Trypanosoma cruzi based on amplicon sequencing of the minicircle hypervariable region.PLoS neglected tropical diseases · 2023Article
- Unveiling challenges in real-time PCR strategies for detecting treatment failure: observations from clinical trials on chronic Chagas disease.Frontiers in parasitology · 2023Review
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- Differential Modulation of Mouse Heart Gene Expression by Infection With TwoFrontiers in genetics · 2020Article
- Elucidating diversity in the class composition of the minicircle hypervariable region of Trypanosoma cruzi: New perspectives on typing and kDNA inheritance.PLoS neglected tropical diseases · 2019Article
- Sexual reproduction in a natural Trypanosoma cruzi population.PLoS neglected tropical diseases · 2019Article
- Article
- Molecular genotyping, diversity studies and high-resolution molecular markers unveiled by microsatellites in Giardia duodenalis.PLoS neglected tropical diseases · 2018Article
- Cardiomyocyte oxidants production may signal to T. cruzi intracellular development.PLoS neglected tropical diseases · 2017Article
- Geographical, landscape and host associations of Trypanosoma cruzi DTUs and lineages.Parasites & vectors · 2016Article
- Characterizing the genetic diversity of the monkey malaria parasite Plasmodium cynomolgi.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2016Article
- Article
- Article
- Identification and Characterization of Microsatellite Markers Derived from the Whole Genome Analysis of Taenia solium.PLoS neglected tropical diseases · 2015Article
- The population genetics of Trypanosoma cruzi revisited in the light of the predominant clonal evolution model.Acta tropica · 2015Article
- Assessment of population genetic structure in the arbovirus vector midge, Culicoides brevitarsis (Diptera: Ceratopogonidae), using multi-locus DNA microsatellites.Veterinary research · 2015Article
- Between a bug and a hard place: Trypanosoma cruzi genetic diversity and the clinical outcomes of Chagas disease.Expert review of anti-infective therapy · 2015Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe here the identification of eight polymorphic microsatellite loci with (CA)n repeats in the Trypanosoma cruzi genome based on the affinity capture of fragments using biotinylated (CA)12 attached to streptavidin-coated magnetic beads. The presence of two peaks in PCR amplification products from individual clones confirmed that T. cruzi is diploid. Hardy-Weinberg and linkage disequilibrium analyses suggested that sexual reproduction is rare or absent and that the population structure is clonal. Several strains, especially those isolated from nonhuman sources, showed more than two alleles in many loci demonstrating that they were multiclonal. The phylogenetic analysis of T. cruzi based on microsatellites revealed a great genetic distance among strains, although the strain dispersion profile in the Wagner network was in general agreement with the species dimorphism found by PCR amplification of the divergent region of the rRNA 24Salpha gene.
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