ArticleProceedings of the National Academy of Sciences of the United States of America1998
Functional association between Arf and RalA in active phospholipase D complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 132 citations in OpenAlex.
- Review
- Article
- Ral GTPases promote breast cancer metastasis by controlling biogenesis and organ targeting of exosomes.eLife · 2021Article
- Amino acid-dependent control of mTORC1 signaling: a variety of regulatory modes.Journal of biomedical science · 2020Review
- Bi-allelic Variants in RALGAPA1 Cause Profound Neurodevelopmental Disability, Muscular Hypotonia, Infantile Spasms, and Feeding Abnormalities.American journal of human genetics · 2020Article
- The Small GTPase Ral orchestrates MVB biogenesis and exosome secretion.Small GTPases · 2018Article
- Phospholipase D-dependent mTOR complex 1 (mTORC1) activation by glutamine.The Journal of biological chemistry · 2018Article
- Multifactorial Regulation of G Protein-Coupled Receptor Endocytosis.Biomolecules & therapeutics · 2017Review
- Ral-Arf6 crosstalk regulates Ral dependent exocyst trafficking and anchorage independent growth signalling.Cellular signalling · 2016Article
- RAL-1 controls multivesicular body biogenesis and exosome secretion.The Journal of cell biology · 2015Article
- Ral small GTPase signaling and oncogenesis: More than just 15minutes of fame.Biochimica et biophysica acta · 2014Review
- Paxillin phosphorylation and complexing with Erk and FAK are regulated by PLD activity in MDA-MB-231 cells.Cellular signalling · 2012Article
- Phospholipase D mediates nutrient input to mammalian target of rapamycin complex 1 (mTORC1).The Journal of biological chemistry · 2011Article
- Role of phospholipase D1 in glucose-induced insulin secretion in pancreatic Beta cells.Experimental & molecular medicine · 2010Article
- Bacterial toxins and the nervous system: neurotoxins and multipotential toxins interacting with neuronal cells.Toxins · 2010Review
- The GTPase RalA regulates different steps of the secretory process in pancreatic beta-cells.PloS one · 2009Article
- Honokiol suppresses survival signals mediated by Ras-dependent phospholipase D activity in human cancer cells.Clinical cancer research : an official journal of the American Association for Cancer Research · 2008Article
- Phospholipase D1 is an effector of Rheb in the mTOR pathway.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Phospholipase D provides a survival signal in human cancer cells with activated H-Ras or K-Ras.Cancer letters · 2007Article
- Ras effector pathways modulate scatter factor-stimulated NF-kappaB signaling and protection against DNA damage.Oncogene · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
Abstract
Activation of phospholipase D1 (PLD1) by Arf has been implicated in vesicle transport and membrane trafficking. PLD1 has also been shown to be associated with the small GTPase RalA, which functions downstream from Ras in a Ras-RalA GTPase cascade that facilitates intracellular signal transduction. Although PLD1 associates directly with RalA, RalA has no effect upon the activity of PLD1. However, PLD1 precipitated from cell lysates with immobilized glutathione S-transferase-RalA fusion protein is active. This suggests the presence of an additional activating factor in the active RalA-PLD1 complexes. Because Arf stimulates PLD1, we looked for the presence of Arf in the active RalA-PLD1 complexes isolated from v-Src- and v-Ras-transformed cell lysates. Low levels of Arf protein were detected in RalA-PLD1 complexes; however, if guanosine 5'-[gamma-thio]triphosphate was added to activate Arf and stimulate translocation to the membrane, high levels of Arf were precipitated by RalA from cell lysates. Interestingly, deletion of 11 amino-terminal amino acids unique to Ral GTPases, which abolished the ability of RalA to precipitate PLD activity, prevented the association between RalA and Arf. Brefeldin A, which inhibits Arf GDP-GTP exchange, inhibited PLD activity in v-Src- and v-Ras-transformed cells but not in the nontransformed cells, suggesting that the association of Arf with RalA is required for the increased PLD activity induced by v-Src and v-Ras. These data implicate Arf in the transduction of intracellular signals activated by v-Src and mediated by the Ras-RalA GTPase cascade. Because both Arf and PLD1 stimulate vesicle formation in the Golgi, these data raise the possibility that vesicle formation and trafficking may play a role in the transduction of intracellular signals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.