ArticleProceedings of the National Academy of Sciences of the United States of America1998
In vitro disassembly and reassembly of an ABC transporter, the histidine permease.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 52 citations in OpenAlex.
- The Shape of Things to Come: α-Helical Membrane Protein Folding on the Ribosome.Chemical reviews · 2026Review
- Metabolomic and metagenomic analyses elucidate the role of intercropping in mitigating continuous cropping challenges in tobacco.Frontiers in plant science · 2024Article
- ATP-Binding Cassette Transporters: Snap-on Complexes?Sub-cellular biochemistry · 2022Article
- Role of cis-trans proline isomerization in the function of pathogenic enterobacterial Periplasmic Binding Proteins.PloS one · 2017Article
- Structural basis for substrate specificity of an amino acid ABC transporter.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes.The Journal of biological chemistry · 2015Article
- Mycobacterium tuberculosis cell division protein, FtsE, is an ATPase in dimeric form.The protein journal · 2015Article
- TcyR regulates L-cystine uptake via the TcyABC transporter in Streptococcus mutans.FEMS microbiology letters · 2012Article
- In vitro folding and assembly of the Escherichia coli ATP-binding cassette transporter, BtuCD.The Journal of biological chemistry · 2011Article
- Article
- Human body temperature (37degrees C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12.Journal of bacteriology · 2007Article
- Functional reassembly of the Escherichia coli maltose transporter following purification of a MalF-MalG subassembly.Journal of bacteriology · 2005Article
- Side chain and backbone contributions of Phe508 to CFTR folding.Nature structural & molecular biology · 2005Article
- Oligomerization and activation of the FliI ATPase central to bacterial flagellum assembly.Molecular microbiology · 2003Article
- Purification and characterization of the membrane-bound complex of an ABC transporter, the histidine permease.Journal of bioenergetics and biomembranes · 2001Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The membrane-bound complex of the Salmonella typhimurium periplasmic histidine permease, a member of the ABC transporters (or traffic ATPases) superfamily, is composed of two integral membrane proteins, HisQ and HisM, and two copies of an ATP-binding subunit, HisP. The complex hydrolyzes ATP upon induction of the activity by the liganded soluble receptor, the periplasmic histidine-binding protein, HisJ. Here we take advantage of the modular organization of this system to show that the nucleotide-binding component can be stripped off the integral membrane components, HisQ and HisM. The complex can be reconstituted by using the HisP-depleted membranes containing HisQ and HisM and pure soluble HisP. We show that HisP has high affinity for the HisP-depleted complex, HisQM, and that two HisP molecules are recruited independently of each other for each HisQM unit. The in vitro reassembled complex has entirely normal properties, responding to HisJ and ATPase inhibitors with the same characteristics as the original complex and in contrast to those of soluble HisP. These results show that HisP is absolutely required for ATP hydrolysis, that HisQM cannot hydrolyze ATP, that HisP depends on HisQM to relay the inducing signal from the soluble receptor, HisJ, and that HisQM regulates the ATPase activity of HisP. We also show that HisP changes conformation upon exposure to phospholipids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.