ArticleProceedings of the National Academy of Sciences of the United States of America1998
Derepression of the C/EBPalpha gene during adipogenesis: identification of AP-2alpha as a repressor.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 87 citations in OpenAlex.
- C/EBPα plays a critical role in adipocyte differentiation and obesity.Frontiers in molecular biosciences · 2026Review
- Deletion of Tfap2a in hepatocytes and macrophages promotes the progression of hepatocellular carcinoma by regulating SREBP1/FASN/ACC pathway and anti-inflammatory effect of IL10.Cell death & disease · 2025Article
- Mechanical overload-induced muscle-derived extracellular vesicles promote adipose tissue lipolysis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Downregulation of SUV39H1 and CITED2 Exerts Additive Effect on Promoting Adipogenic Commitment of Human Mesenchymal Stem Cells.Stem cells and development · 2021Article
- Protein kinase D up-regulates transcription ofThe Journal of biological chemistry · 2019Article
- KCTD: A new gene family involved in neurodevelopmental and neuropsychiatric disorders.CNS neuroscience & therapeutics · 2019Review
- Epigenetic programming of Dnmt3a mediated by AP2α is required for granting preadipocyte the ability to differentiate.Cell death & disease · 2016Article
- Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest.The International journal of developmental biology · 2016Article
- Suv39h1 mediates AP-2α-dependent inhibition of C/EBPα expression during adipogenesis.Molecular and cellular biology · 2014Article
- Inhibition of neural crest formation by Kctd15 involves regulation of transcription factor AP-2.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Alternative TFAP2A isoforms have distinct activities in breast cancer.Breast cancer research : BCR · 2011Article
- Involvement of cytoskeleton-associated proteins in the commitment of C3H10T1/2 pluripotent stem cells to adipocyte lineage induced by BMP2/4.Molecular & cellular proteomics : MCP · 2011Article
- Protein kinase Cdelta stimulates proteasome-dependent degradation of C/EBPalpha during apoptosis induction of leukemic cells.PloS one · 2009Article
- Chromatin immunoprecipitation on microarray analysis of Smad2/3 binding sites reveals roles of ETS1 and TFAP2A in transforming growth factor beta signaling.Molecular and cellular biology · 2009Article
- Temporal and functional profile of the transcriptional regulatory network in the early regenerative response to partial hepatectomy in the rat.BMC genomics · 2008Article
- AP-2 factors act in concert with Notch to orchestrate terminal differentiation in skin epidermis.The Journal of cell biology · 2008Article
- Article
- CCAAT/enhancer-binding proteins: structure, function and regulation.The Biochemical journal · 2002Review
- Analysis of the Xenopus laevis CCAAT-enhancer binding protein alpha gene promoter demonstrates species-specific differences in the mechanisms for both auto-activation and regulation by Sp1.Nucleic acids research · 2001Article
- Sequential repression and activation of the CCAAT enhancer-binding protein-alpha (C/EBPalpha ) gene during adipogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
During adipogenesis, CCAAT/enhancer binding protein alpha (C/EBPalpha) serves as a pleiotropic transcriptional activator of adipocyte genes. Previously, we identified dual repressive elements in the C/EBPalpha gene and a putative transacting factor (C/EBPalpha undifferentiated protein, or CUP) expressed by preadipocytes, but not adipocytes, that bind to these elements. In the present investigation, CUP was purified 17,000-fold from nuclear extracts of 3T3-L1 preadipocytes. Amino acid sequence and mass spectral analysis of tryptic peptides derived from purifed CUP (molecular mass approximately 50 kDa) revealed that the repressor is (or contains) an isoform of the transcription factor, AP-2alpha. Electrophoretic mobility shift and Western blot analysis on purified CUP and preadipocyte nuclear extracts confirmed the identity of CUP as AP-2alpha. Both AP-2alpha protein and CUP binding activity are expressed by preadipocytes and then decrease concomitantly during differentiation of 3T3-L1 preadipocytes into adipocytes. Consistent with a repressive role of AP-2alpha/CUP, an AP-2alpha1 expression vector, cotransfected with a C/EBPalpha promoter-reporter construct into 3T3-L1 adipocytes, inhibited reporter gene transcription. Taken together with previous results, these findings suggest that in preadipocytes the C/EBPalpha gene is repressed by AP-2alpha/CUP, which, upon induction of differentiation, is down-regulated, allowing expression of the gene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.