ArticleThe Journal of physiology1998
Non-specificity of chloride channel blockers in rat cerebral arteries: block of the L-type calcium channel.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 84 citations in OpenAlex.
- A cryptic-site ligand stabilizes a non-canonical interface and blocks membrane insertion of the chloride intracellular channel CLIC1.The Journal of biological chemistry · 2026Article
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Chloride Ions, Vascular Function and Hypertension.Biomedicines · 2022Review
- Positive modulation of the TMEM16B mediated currents by TRPV4 antagonist.Biochemistry and biophysics reports · 2021Article
- Intracellular Chloride Channels: Novel Biomarkers in Diseases.Frontiers in physiology · 2020Review
- Chloride channel blocker IAA-94 increases myocardial infarction by reducing calcium retention capacity of the cardiac mitochondria.Life sciences · 2019Article
- Inhibitors of connexin and pannexin channels as potential therapeutics.Pharmacology & therapeutics · 2017Review
- Calcium dynamics underlying the myogenic response of the renal afferent arteriole.American journal of physiology. Renal physiology · 2014Article
- Contribution of Ca²⁺-dependent Cl⁻ channels to norepinephrine-induced contraction of femoral artery is replaced by increasing EDCF contribution during ageing.BioMed research international · 2014Article
- Regulation of cerebral artery smooth muscle membrane potential by Ca²⁺-activated cation channels.Microcirculation (New York, N.Y. : 1994) · 2013Review
- The ClC-3 chloride channels in cardiovascular disease.Acta pharmacologica Sinica · 2011Review
- Biophysically based modeling of the interstitial cells of cajal: current status and future perspectives.Frontiers in physiology · 2011Article
- Article
- Ca2+-activated Cl- current in retinal arteriolar smooth muscle.Investigative ophthalmology & visual science · 2009Article
- Specific inhibition of the plasmodial surface anion channel by dantrolene.Eukaryotic cell · 2006Article
- Monitoring Cl- movement in single cells exposed to hypotonic solution.The Journal of membrane biology · 2005Article
- Effects of chloride and potassium channel blockers on apoptotic cell shrinkage and apoptosis in cortical neurons.Pflugers Archiv : European journal of physiology · 2004Article
- Effects of chloride channel blockers on hypotonicity-induced contractions of the rat trachea.British journal of pharmacology · 2004Article
- The Cl(-) channel blocker niflumic acid releases Ca(2+) from an intracellular store in rat pulmonary artery smooth muscle cells.British journal of pharmacology · 2003Article
- Effects of osmotic changes on the chemoreceptor cell of rat carotid body.The Journal of physiology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The effects of chloride channel blockers on pressure-induced constriction, K(+)-induced force, and whole-cell calcium channel currents were tested in rat cerebral arteries using isobaric and isometric myography, and patch clamp. 2. Under isobaric conditions at 75 mmHg, 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), a chloride channel blocker, reversibly depressed the myogenic constriction with an IC50 of 32.8 +/- 0.52 microM (mean +/- S.E.M., n = 5). Blockers of Ca(2+)-activated chloride channels, flufenamic acid (100 microM) and 9-anthracene chloride (9-AC; 1 mM), and the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel blocker, glibenclamide (100 microM), were without effect in this tissue (n = 3). 3. Under isobaric conditions at 20 mmHg, 37 degrees C, raising [K+]o to 45 mM induced a constriction which was unaffected by 100 microM NPPB (n = 4). In contrast, at 75 mmHg and 18-21 degrees C, 100 microM NPPB completely and reversibly blocked a 45 mM K(+)-induced constriction (n = 3). 4. Under isometric conditions, NPPB reversibly depressed a 45 mM K(+)-induced force with an IC50 of 10.0 +/- 0.76 microM (mean +/- S.E.M., n = 5). Indanyloxyacetic acid 94 (IAA-94), another chloride channel blocker, depressed the K(+)-induced force with an IC50 of 17.0 +/- 1.2 microM (mean +/- S.E.M., n = 4). 5. Using whole-cell patch clamp, 100 microM NPPB or 200 microM IAA-94 blocked calcium channel currents carried by 10 mM Ba2+ by 79.1 +/- 1.7 and 39.8 +/- 7.0%, respectively (mean +/- S.E.M., n = 6). 6. In summary, chloride channel blockers depress calcium channel currents in rat cerebral arteries, which could contribute to a reduction in myogenic contraction.
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