ArticleNucleic acids research1998
NMR evidence for a base triple in the HIV-2 TAR C-G.C+ mutant-argininamide complex.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Thermodynamic prediction of RNA cellular activity from sequence via conformational ensembles.Cell · 2026Article
- Progress in 7SK ribonucleoprotein structural biology.Frontiers in molecular biosciences · 2023Review
- Exposing Hidden High-Affinity RNA Conformational States.Journal of the American Chemical Society · 2020Article
- Adenine protonation enables cyclic-di-GMP binding to cyclic-GAMP sensing riboswitches.RNA (New York, N.Y.) · 2018Article
- hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA.Nucleic acids research · 2016Article
- Building a stable RNA U-turn with a protonated cytidine.RNA (New York, N.Y.) · 2014Article
- Inhibition of Tat-mediated HIV-1 replication and neurotoxicity by novel GSK3-beta inhibitors.Virology · 2011Article
- Mechanical unfolding of RNA: from hairpins to structures with internal multiloops.Biophysical journal · 2007Article
- New, extended hairpin form of the TAR-2 RNA domain points to the structural polymorphism at the 5' end of the HIV-2 leader RNA.Nucleic acids research · 2006Article
- Evidence for a base triple in the free HIV-1 TAR RNA.RNA (New York, N.Y.) · 2004Article
- NCIR: a database of non-canonical interactions in known RNA structures.Nucleic acids research · 2002Article
- A protonated base pair participating in rRNA tertiary structural interactions.Nucleic acids research · 2001Article
- Molecular dynamics studies of the HIV-1 TAR and its complex with argininamide.Nucleic acids research · 2000Article
- The human immunodeficiency virus type 1 TAR RNA upper stem-loop plays distinct roles in reverse transcription and RNA packaging.Journal of virology · 2000Article
- Detection of N-H...N hydrogen bonding in RNA via scalar couplings in the absence of observable imino proton resonances.Nucleic acids research · 2000Article
- An important base triple anchors the substrate helix recognition surface within the Tetrahymena ribozyme active site.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Formation of a specific complex between the HIV Tat protein and the small RNA element TAR is critical for activation of viral transcription. A model complex for this interaction composed of HIV-2 TAR and the amide derivative of arginine has been developed to study how Tat and TAR interact specifically. We have previously determined a high resolution NMR structure of the HIV-2 TAR-argininamide complex. The argininamide guanidium group hydrogen bonds to the major groove face of G26 and is stacked between U23 and A22, forming an arginine sandwich. This structure also provided evidence for formation of a U38-A27.U38 base triple, as U23 is positioned in the major groove within hydrogen bonding distance to A27. However, the expected U23 imino proton was not observed, preventing unambiguous identification of the base triple. Previous work on an isomorphic C38-G27.C23+ base triple mutant of the three base bulge HIV-1 TAR-argininamide complex demonstrated that the base triple is required for specific argininamide binding. Here we investigate the same C38-G27.C23+ base triple mutant in the context of two base bulge HIV-2 TAR. The improved NMR spectral properties of HIV-2 TAR allowed observation of the C23 amino and imino protons for the first time, providing direct evidence that a hydrogen bonding interaction is occurring. The NOEs observed correspond to those observed in the high resolution structure of the HIV-2 TAR-argininamide complex, confirming that a base triple is an important feature of the TAR-argininamide interaction.
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