ArticleNucleic acids research1998
An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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Who cites it
91 citing papers in PubMed.
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- Molecular Mechanism of Regulation of the Purine Salvage Enzyme XPRT by the Alarmones pppGpp, ppGpp, and pGpp.Journal of molecular biology · 2020Article
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- Evolution of (p)ppGpp-HPRT regulation through diversification of an allosteric oligomeric interaction.eLife · 2019Article
- Kinetic and catalytic properties of M.HpyAXVII, a phase-variable DNA methyltransferase fromThe Journal of biological chemistry · 2019Article
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- Loss-of-function of Nav1.8/D1639N linked to human pain can be rescued by lidocaine.Pflugers Archiv : European journal of physiology · 2018Article
- Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy.Applied microbiology and biotechnology · 2018Article
- The ASIC3/P2X3 cognate receptor is a pain-relevant and ligand-gated cationic channel.Nature communications · 2018Article
- Regulation of HThe Journal of membrane biology · 2018Article
- Improving 10-deacetylbaccatin III-10-β-O-acetyltransferase catalytic fitness for Taxol production.Nature communications · 2017Article
31 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The QuikChangeTM protocol is one of the simplest and fastest methods for site-directed mutagenesis, but introduces mutations at only one site at a time, and requires two HPLC-purified complementary oligonucleotides. Here, we describe that this method can be used with non-overlapping oligonucleotides. By doing this, two separate sites can be mutagenised simultaneously, or money can be saved by using a second 'standard' oligonucleotide. By a further modification, we have also used the QuikChangeTM approach to exchange DNA sequences between closely related genes.
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