ArticleNucleic acids research1998
Interaction between the N-terminus of human topoisomerase I and SV40 large T antigen.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 38 citations in OpenAlex.
- The prolyl isomerase, FKBP25, interacts with RNA-engaged nucleolin and the pre-60S ribosomal subunit.RNA (New York, N.Y.) · 2014Article
- Specific domains of nucleolin interact with Hdm2 and antagonize Hdm2-mediated p53 ubiquitination.The FEBS journal · 2012Article
- Novel checkpoint response to genotoxic stress mediated by nucleolin-replication protein a complex formation.Molecular and cellular biology · 2005Article
- The cap region of topoisomerase I binds to sites near both ends of simian virus 40 T antigen.Journal of virology · 2003Article
- Bidirectional DNA unwinding by a ternary complex of T antigen, nucleolin and topoisomerase I.EMBO reports · 2003Article
- The tumor suppressor protein p53 stimulates the formation of the human topoisomerase I double cleavage complex in vitro.Oncogene · 2002Article
- Stress-dependent nucleolin mobilization mediated by p53-nucleolin complex formation.Molecular and cellular biology · 2002Article
- Characterization of BTBD1 and BTBD2, two similar BTB-domain-containing Kelch-like proteins that interact with Topoisomerase I.BMC genomics · 2002Article
- Topoisomerase I associates specifically with simian virus 40 large-T-antigen double hexamer-origin complexes.Journal of virology · 2000Article
- Human topoisomerase I promotes initiation of simian virus 40 DNA replication in vitro.Molecular and cellular biology · 1999Article
- Targeting to transcriptionally active loci by the hydrophilic N-terminal domain of Drosophila DNA topoisomerase I.Molecular and cellular biology · 1998Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We have attempted to identify human topoisomerase I-binding proteins in order to gain information regarding the cellular roles of this protein and the cytotoxic mechanisms of the anticancer drug camptothecin, which specifically targets topoisomerase I. In the course of this work we identified an interaction between the N-terminus of human topoisomerase I and the SV40 T antigen that is detectable in vitro using both affinity chromatography and co-immunoprecipitation. Additional results indicate that this interaction does not require intermediary DNA or stoichiometric quantities of other proteins. Furthermore, the interaction is detectable in vivo using a yeast two-hybrid assay. Two binding sites for T antigen are apparent on the topoisomerase I protein: one consisting of amino acids 1-139, the other present in the 383-765 region of the protein. Interestingly, nucleolin, which binds the 166-210 region of topoisomerase I, is able to bind an N-terminal fragment of topoisomerase I concurrently with T antigen. Taken together with our prior identification of nucleolin as a topoisomerase I-binding protein, the current results suggest that helicase-binding is a major role of the N-terminus of human topoisomerase I and that the resultant helicase-topoisomerase complex may function as a eukaryotic gyrase.
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