ArticleNucleic acids research1998
Dimerization of the testis brain RNA-binding protein (translin) is mediated through its C-terminus and is required for DNA- and RNA-binding.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 45 citations in OpenAlex.
- Conformational transitions in human translin enable nucleic acid binding.Nucleic acids research · 2013Article
- Molecular evolution of translin superfamily proteins within the genomes of eubacteria, archaea and eukaryotes.Journal of molecular evolution · 2012Article
- The translin-TRAX complex (C3PO) is a ribonuclease in tRNA processing.Nature structural & molecular biology · 2012Article
- Multimeric assembly and biochemical characterization of the Trax-translin endonuclease complex.Nature structural & molecular biology · 2011Article
- Analysis of nucleic acid binding by a recombinant translin-trax complex.Biochemical and biophysical research communications · 2010Article
- The Translin/Trax RNA binding complex: clues to function in the nervous system.Biochimica et biophysica acta · 2008Review
- Cloning and characterization of the Schizosaccharomyces pombe homologs of the human protein Translin and the Translin-associated protein TRAX.Nucleic acids research · 2005Article
- RNA-protein interactions promote asymmetric sorting of the ASH1 mRNA ribonucleoprotein complex.RNA (New York, N.Y.) · 2003Article
- Mice deficient for testis-brain RNA-binding protein exhibit a coordinate loss of TRAX, reduced fertility, altered gene expression in the brain, and behavioral changes.Molecular and cellular biology · 2003Article
- A sperm-associated WD repeat protein orthologous to Chlamydomonas PF20 associates with Spag6, the mammalian orthologue of Chlamydomonas PF16.Molecular and cellular biology · 2002Article
- Candidate RNA-binding proteins regulating extrasomatic mRNA targeting and translation in mammalian neurons.Molecular neurobiology · 2002Review
- Altering the GTP binding site of the DNA/RNA-binding protein, Translin/TB-RBP, decreases RNA binding and may create a dominant negative phenotype.Nucleic acids research · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Testis brain-RNA-binding protein (TB-RBP) is a single-stranded DNA- and RNA-binding protein that is involved in chromosomal translocations, mRNA transport and translational regulation. Here we show from in vitro and in vivo protein binding studies that TB-RBP dimers are the minimum structural unit needed for DNA- and RNA-binding. Truncation studies demonstrate that the C-terminus of 55 amino acids of TB-RBP is essential, but not sufficient for DNA- or RNA-binding, and deletion of the leucine zipper motif in the C-terminus abolishes DNA- and RNA-binding. Changing cysteine 225 in the C-terminus to alanine does not significantly reduce DNA- or RNA-binding, but reduces the stability of the dimer. We conclude that the leucine zipper motif is required to maintain two molecules of TB-RBP as a dimer which is stabilized by a disulfide bond involving cysteine 225.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.