ArticleRNA (New York, N.Y.)1998
Transcription termination downstream of the Saccharomyces cerevisiae FBP1 [changed from FPB1] poly(A) site does not depend on efficient 3'end processing.
Article in RNA (New York, N.Y.), 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- Genetic manipulation of longevity-related genes as a tool to regulate yeast life span and metabolite production during winemaking.Microbial cell factories · 2013Article
- A sequence element downstream of the yeast HTB1 gene contributes to mRNA 3' processing and cell cycle regulation.Molecular and cellular biology · 2002Article
- Transcriptional collision between convergent genes in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Rna15 interaction with the A-rich yeast polyadenylation signal is an essential step in mRNA 3'-end formation.Molecular and cellular biology · 2001Article
- Mechanism of poly(A) signal transduction to RNA polymerase II in vitro.Molecular and cellular biology · 2001Article
- Frequent homologous recombination events between molecules of one RNA component in a multipartite RNA virus.Journal of virology · 2000Article
- Statistical analysis of yeast genomic downstream sequences reveals putative polyadenylation signals.Nucleic acids research · 2000Article
- Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.Microbiology and molecular biology reviews : MMBR · 1999Review
- Definition of transcriptional pause elements in fission yeast.Molecular and cellular biology · 1999Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
Efficient transcription termination downstream of poly(A) sites has been shown to correlate with the strength of an upstream polyadenylation signal and the presence of a polymerase pause site. To further investigate the mechanism linking termination with 3'-end processing, we analyzed the cis-acting elements that contribute to these events in the Saccharomyces cerevisiae FBP1 gene. FBP1 has a complex polyadenylation signal, and at least three efficiency elements must be present for efficient processing. However, not all combinations of these elements are equally effective. This gene also shows a novel organization of sequence elements. A strong positioning element is located upstream, rather than downstream, of the efficiency elements, and functions to select the cleavage site in vitro and in vivo. Transcription run-on analysis indicated that termination occurs within 61 nt past the poly(A) site. Deletion of two UAUAUA-type efficiency elements greatly reduces polyadenylation in vivo and in vitro, but transcription termination is still efficient, implying that FBP1 termination signals may be distinct from those for polyadenylation. Alternatively, assembly of a partial, but nonfunctional, polyadenylation complex on the nascent transcript may be sufficient to cause termination.
Indexed as
Identifiers
9510332PMC1369619What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.