ArticleThe Journal of physiology1998
Ca2+ permeability of mouse and chick nicotinic acetylcholine receptors expressed in transiently transfected human cells.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 65 citations in OpenAlex.
- Reduction of the CaFrontiers in cellular neuroscience · 2025Review
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- Donepezil inhibits neuromuscular junctional acetylcholinesterase and enhances synaptic transmission and function in isolated skeletal muscle.British journal of pharmacology · 2022Article
- "Calcium bombs" as harbingers of synaptic pathology and their mitigation by magnesium at murine neuromuscular junctions.Frontiers in molecular neuroscience · 2022Article
- Article
- Nicotinic Acetylcholine Receptor Involvement in Inflammatory Bowel Disease and Interactions with Gut Microbiota.International journal of environmental research and public health · 2021Review
- The Structure, Function, and Physiology of the Fetal and Adult Acetylcholine Receptor in Muscle.Frontiers in molecular neuroscience · 2020Review
- The Distribution of Charged Amino Acid Residues and the CaFrontiers in molecular neuroscience · 2017Article
- The habenulo-interpeduncular pathway in nicotine aversion and withdrawal.Neuropharmacology · 2015Review
- A mutation in the extracellular domain of the α7 nAChR reduces calcium permeability.Pflugers Archiv : European journal of physiology · 2014Article
- Cellular, molecular, and genetic substrates underlying the impact of nicotine on learning.Neurobiology of learning and memory · 2014Review
- Neuroprotection of rat retinal ganglion cells mediated through alpha7 nicotinic acetylcholine receptors.Neuroscience · 2013Article
- Phylogenetic differences in calcium permeability of the auditory hair cell cholinergic nicotinic receptor.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Nerve terminal nicotinic acetylcholine receptors initiate quantal GABA release from perisomatic interneurons by activating axonal T-type (Cav3) Ca²⁺ channels and Ca²⁺ release from stores.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Evaluation of Ca2+ permeability of nicotinic acetylcholine receptors in hypothalamic histaminergic neurons.Acta biochimica et biophysica Sinica · 2010Article
- About a new method to measure fractional Ca2+ currents through ligand-gated ion channels.The Journal of general physiology · 2009Article
- Cellular events in nicotine addiction.Seminars in cell & developmental biology · 2009Review
- Nicotine-induced Ca2+-myristoyl switch of neuronal Ca2+ sensor VILIP-1 in hippocampal neurons: a possible crosstalk mechanism for nicotinic receptors.Cellular and molecular neurobiology · 2009Article
- Decremental response to high-frequency trains of acetylcholine pulses but unaltered fractional Ca2+ currents in a panel of "slow-channel syndrome" nicotinic receptor mutants.The Journal of general physiology · 2009Article
- Pathogenic point mutations in a transmembrane domain of the epsilon subunit increase the Ca2+ permeability of the human endplate ACh receptor.The Journal of physiology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Combinations of cDNAs encoding mouse and chick nicotinic acetylcholine receptor (nAChR) subunits were transiently transfected into human BOSC 23 cells, and the expressed receptors were studied by simultaneously recording transmembrane currents and fluorescence transients using the whole-cell patch-clamp technique, and confocal microscopy with the Ca2+ indicator dye fluo-3. 2. The fractional Ca2+ current, Pf, of nAChRs was evaluated as the normalized ratio of nicotine-evoked fluorescence transient over total charge entering the cell (F/Q ratio). Mouse fetal muscle nAChR channels had a Pf, alphabetagammadelta value of 2.1 %. The substitution of the gamma subunit with the epsilon subunit resulted in a 2-fold increase in Pf (4.2 %). The difference in Ca2+ permeability was confirmed by determination of Ca2+/Cs+ permeability ratios. 3. Among the chick neuronal nAChRs tested, Pf,alpha3beta4 was 4.6 %, while Pf, alpha4beta4 and Pf,alpha4beta2 were 3.0 % and 2.9 %, respectively. 4. The amplitude of the current elicited by the activation of alpha3beta4 nAChRs increased as the external Ca2+ concentration was raised from 2 to 110 mM, whereas currents flowing through all other nAChRs tested were reduced to various extents. 5. Our findings indicate that the adult-type muscle nAChR (alphabetaepsilondelta) is more permeable to Ca2+ than the fetal-type (alphabetagammadelta), while ganglionic-like alpha3beta4 nAChR is more permeable to Ca2+ than the examined alpha4-containing nAChRs. The functional significance is discussed.
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