ArticleThe Journal of physiology1998
Variability in spontaneous subcellular calcium release in guinea-pig ileum smooth muscle cells.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 98 citations in OpenAlex.
- Reactive Oxygen Species (ROS) in Metabolic Disease-Don't Shoot the Metabolic Messenger.International journal of molecular sciences · 2025Review
- CaPhysiological reviews · 2024Review
- On a Magical Mystery Tour with 8-Bromo-Cyclic ADP-Ribose: From All-or-None Block to Nanojunctions and the Cell-Wide Web.Molecules (Basel, Switzerland) · 2020Review
- CaThe Journal of physiology · 2019Article
- Size Matters: Ryanodine Receptor Cluster Size Heterogeneity Potentiates Calcium Waves.Biophysical journal · 2019Article
- Subplasma membrane Ca2+ signals.IUBMB life · 2012Review
- Relationship between Ca2+ sparklets and sarcoplasmic reticulum Ca2+ load and release in rat cerebral arterial smooth muscle.American journal of physiology. Heart and circulatory physiology · 2011Article
- Calcium signaling in smooth muscle.Cold Spring Harbor perspectives in biology · 2011Review
- Calcium sparks in the intact gerbil spiral modiolar artery.BMC physiology · 2011Article
- Regulation of gastrointestinal motility by Ca2+/calmodulin-stimulated protein kinase II.Archives of biochemistry and biophysics · 2011Review
- Modulation of murine gastric antrum smooth muscle STOC activity and excitability by phospholamban.The Journal of physiology · 2008Article
- Regulation of smooth muscle excitation and contraction.Neurogastroenterology and motility · 2008Review
- Article
- Role of Ca2+ entry and Ca2+ stores in atypical smooth muscle cell autorhythmicity in the mouse renal pelvis.British journal of pharmacology · 2007Article
- Mini-dystrophin expression down-regulates IP3-mediated calcium release events in resting dystrophin-deficient muscle cells.The Journal of general physiology · 2006Article
- Ca2+ -induced Ca2+ release through localized Ca2+ uncaging in smooth muscle.The Journal of general physiology · 2006Article
- On the loose: uncaging Ca2+ -induced Ca2+ release in smooth muscle.The Journal of general physiology · 2006Article
- Constitutively active L-type Ca2+ channels.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Identification and spatiotemporal characterization of spontaneous Ca2+ sparks and global Ca2+ oscillations in retinal arteriolar smooth muscle cells.Investigative ophthalmology & visual science · 2004Article
- Organization of Ca2+ release units in excitable smooth muscle of the guinea-pig urinary bladder.Biophysical journal · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
1. Spontaneous, localized transient increases in [Ca2+]i ('Ca2+ sparks') were observed in about 40 % of fluo-3-loaded myocytes examined using laser scanning confocal microscopy. Ca2+ sparks persisted after application of Cd2+ (200 microM), but were abolished by ryanodine (30 microM) or thapsigargin (0.1 microM), suggesting that they arise from the spontaneous activation of ryanodine receptors (RyR) in the sarcoplasmic reticulum (SR). 2. Ca2+ sparks occurred much more frequently at certain sites (or 'frequent discharge sites', FDSs) within any confocal plane of the cell and line-scan imaging revealed a wide variation in their spatial size, amplitude and time course. Some spontaneous local transients were very similar to 'Ca2+ sparks' observed in heart, i.e. lasting approximately 200 ms with a peak fluorescence ratio of 1.75 +/- 0.23 (mean +/- s.d., n = 33). Other events were faster and smaller, lasting only approximately 40 ms with a peak normalized fluorescence of 1.36 +/- 0.09 (mean +/- s.d., n = 28). 3. Spontaneous Ca2+ waves with a wide range of propagation velocities (between 30 and 260 micron s-1) were also observed. In about 60 % of records (n = 33), Ca2+ sparks could be detected at the sites of wave initiation. Waves of elevated [Ca2+]i propagated with non-constant velocity and in some cases terminated. These observations could be explained by heterogeneity in the distribution of subcellular release sites as well as variability in the contribution of each release site to the wave. 4. Spontaneous [Ca2+]i transients in single dispersed visceral smooth muscle cells have a wide spectrum of behaviour that is likely to be the result of spatio-temporal recruitment of smaller local events, probably via a calcium-induced calcium release (CICR) mechanism. The spatial non-uniformity of SR and RyR distribution within the cell may account for the existence of 'frequent discharge sites' firing the majority of the smooth muscle Ca2+ sparks and the wide variation in the Ca2+ wave propagation velocities observed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.