ArticleJournal of clinical microbiology1998
Electrophoretic karyotypes and genome sizing of the pathogenic fungus Paracoccidioides brasiliensis.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 49 citations in OpenAlex.
- Telomeric assemblies ofbioRxiv : the preprint server for biology · 2025Article
- Updates inJournal of fungi (Basel, Switzerland) · 2021Review
- Molecular and morphological data support the existence of a sexual cycle in species of the genus Paracoccidioides.Eukaryotic cell · 2013Article
- Endophytic life strategies decoded by genome and transcriptome analyses of the mutualistic root symbiont Piriformospora indica.PLoS pathogens · 2011Article
- Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.PloS one · 2011Article
- Identification and characterization of Tc1/mariner-like DNA transposons in genomes of the pathogenic fungi of the Paracoccidioides species complex.BMC genomics · 2010Article
- Genomic DNA microarray comparison of gene expression patterns in Paracoccidioides brasiliensis mycelia and yeasts in vitro.Microbiology (Reading, England) · 2009Article
- Cell organisation, sulphur metabolism and ion transport-related genes are differentially expressed in Paracoccidioides brasiliensis mycelium and yeast cells.BMC genomics · 2006Article
- Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: identification of putative homologues of Candida albicans virulence and pathogenicity genes.Eukaryotic cell · 2003Article
- Polymorphism in the gene coding for the immunodominant antigen gp43 from the pathogenic fungus Paracoccidioides brasiliensis.Journal of clinical microbiology · 2000Article
- Developments in fungal taxonomy.Clinical microbiology reviews · 1999Review
- Review
- Insights into the pathobiology of Paracoccidioides brasiliensis from transcriptome analysis--advances and perspectives.MycopathologiaReview
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we present the karyotype analysis and genome sizing of Paracoccidioides brasiliensis, a pathogen refractory to conventional genetic analysis. We have established pulsed-field gel electrophoresis (PFGE) conditions to resolve the high-molecular-weight chromosomal bands of two clinical isolates of P. brasiliensis. Both isolates showed four megabase-sized bands, ranging from 2.0 to 10.0 Mbp. Significant differences in chromosome sizes and in the chromosomal location of genes for the gp43 antigen and chitin synthase were found. Different technical approaches were employed to estimate the DNA content and to define the ploidy of P. brasiliensis. An estimated genome size in the range of 45.7 to 60.9 Mbp was provided by the analysis of data generated by measuring the amplitude of fluorescence intensity of DAPI (4',6-diamidino-2-phenylindole)-stained nuclei (by confocal microscopy). The nuclear genome size estimated by confocal microscopy is twice that estimated by the average sum of the molecular weight of chromosome-sized DNA molecules by PFGE, suggesting that each separated P. brasiliensis chromosomal band is diploid.
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